Non-Metastatic Cells 3 Active Protein | NME3 active protein
Recombinant Human Non-Metastatic Cells 3, BioActive
Gene Names
NME3; NDPKC; NDPK-C; NM23H3; DR-nm23; NM23-H3; c371H6.2
Purity
Greater than 95.0% as determined by SDS-PAGE
Synonyms
Non-Metastatic Cells 3; Recombinant Human Non-Metastatic Cells 3; BioActive; NME3 Active; NME3 Human Active; Non-Metastatic Cells 3 Human Recombinant; Nucleoside diphosphate kinase 3; c371H62; DR-nm23; KIAA0516; NDPK-C; NDPKC; NM23-H3; NM23H3; NME3 active protein
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE
Form/Format
Sterile filtered colorless solution.
The NME3 solution (0.5mg/ml) contains 50% glycerol, 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl and 2mM DTT.
The NME3 solution (0.5mg/ml) contains 50% glycerol, 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl and 2mM DTT.
Sequence
MGSSHHHHHH SSGLVPRGSH MERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE
Biological Activity
Specific activity is >150unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH7.5 at 25 degree C in a couple system with PK/LDH.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Store, frozen at -20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Related Product Information for NME3 active protein
Non-Metastatic Cells 3 or NME3 is a possible suppressor of metastasis. NME3 can be found at a lower levels in greatly metastatic cells when compared to cells that has less metastatic potential NME3 is crucial for the nucleoside triphosphates synthesis, also, in apoptosis induction & hematopoiesis. NME3 is can be expressed during early stages of myeloid differentiation of greatly purified CD34+ cells.
NME3 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 169 amino acids (22-169 a.a.) and having a molecular mass of 19.1kDa.NME3 is fused to a 21 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.
NME3 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 169 amino acids (22-169 a.a.) and having a molecular mass of 19.1kDa.NME3 is fused to a 21 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.
Product Categories/Family for NME3 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
169
NCBI Official Full Name
Nucleoside diphosphate kinase 3
NCBI Official Synonym Full Names
NME/NM23 nucleoside diphosphate kinase 3
NCBI Official Symbol
NME3
NCBI Official Synonym Symbols
NDPKC; NDPK-C; NM23H3; DR-nm23; NM23-H3; c371H6.2
NCBI Protein Information
nucleoside diphosphate kinase 3; NDK 3; NDP kinase 3; NDP kinase C; nucleoside diphosphate kinase C; non-metastatic cells 3, protein expressed in
UniProt Protein Name
Nucleoside diphosphate kinase 3
Protein Family
UniProt Gene Name
NME3
UniProt Synonym Gene Names
NDK 3; NDP kinase 3; NDPKC
UniProt Entry Name
NDK3_HUMAN
Uniprot Description
NME3: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Probably has a role in normal hematopoiesis by inhibition of granulocyte differentiation and induction of apoptosis. Belongs to the NDK family.
Protein type: Kinase, other; Kinase, nucleoside diphosphate; Nucleotide Metabolism - pyrimidine; EC 2.7.4.6; Apoptosis; Motility/polarity/chemotaxis; Nucleotide Metabolism - purine; Other group; NDK family
Chromosomal Location of Human Ortholog: 16q13.3
Cellular Component: mitochondrion; cytosol
Molecular Function: metal ion binding; nucleoside diphosphate kinase activity; ATP binding
Biological Process: GTP biosynthetic process; regulation of apoptosis; CTP biosynthetic process; apoptosis; pyrimidine nucleotide metabolic process; UTP biosynthetic process; nucleoside triphosphate biosynthetic process; nucleoside diphosphate phosphorylation; purine nucleotide metabolic process
Research Articles on NME3
1. Down-regulated DR-nm23 expression is associated with invasion and metastasis in colorectal cancer.
Similar Products
Product Notes
The NME3 nme3 (Catalog #AAA141637) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE. It is sometimes possible for the material contained within the vial of "Non-Metastatic Cells 3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
