PAI-1 native protein

Rat PAI-1 NBD labeled at the reactive center loop

Cat. Num. AAA135964

• Gene Names: Serpine1; Pai1; PAI1A; Planh; Pai1aa; RATPAI1A
• Purity: >95% by SDS-PAGE analysis
• Synonyms: PAI-1; Rat PAI-1 NBD labeled at the reactive center loop; PAI-1 native protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE analysis
• Form/Format: Frozen liquid
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 402

• Extinction Coefficient: 1
• Buffer: 0.05M Sodium Acetate; 0.1M NaCl; 1mM EDTA; pH 5.0
• Preparation and Storage: Recommended Storage: At -70 degree C; Protect from light; Minimum Shelf Life: 3 years from delivery; Shipping Condition: Dry ice

Related Product Information for PAI-1 native protein

P9-NBD Rat PAI-1 was created by mutagenesis of the P9 serine residue (Ser339) on the reactive center loop to cysteine. This provides a free thiol group for incorporation of N,N'-dimethyl-N-(acetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) (NBD), a fluorescent probe highly sensitive to changes in solvation and hydrophobic environment. The fluorescence emission of P9-NBD PAI-1 is enhanced 6-7 fold upon insertion of the reactive center loop into beta-sheet A following complex formation with proteinases, formation of the latent species, or cleavage by elastase. The incorporated probe is excited at 480 nm and displays a broad emission spectrum with a peak centered 542 nm with a resultant blue-shift to 520 nm following reactive center loop insertion. This unique product is an ideal reagent for researchers studying PAI-1 behavior in rat models.

Product Categories/Family for PAI-1 native protein

PAI-1

NCBI and Uniprot Product Information

• NCBI GI #: 6981332
• NCBI GeneID: 24617
• NCBI Accession #: NP_036752.1
• NCBI GenBank Nucleotide #: NM_012620.1
• UniProt Accession #: P20961
• Molecular Weight: 43000
• NCBI Official Full Name: plasminogen activator inhibitor 1
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• NCBI Official Symbol: Serpine1
• NCBI Official Synonym Symbols: Pai1; PAI1A; Planh; Pai1aa; RATPAI1A
• NCBI Protein Information: plasminogen activator inhibitor 1; PAI; PAI-1; serpin E1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, member 1; serine (or cysteine) peptidase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• UniProt Protein Name: Plasminogen activator inhibitor 1
• UniProt Gene Name: Serpine1
• UniProt Synonym Gene Names: Pai1; Planh1; PAI; PAI-1
• UniProt Entry Name: PAI1_RAT

NCBI Description

mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator; may promote neuronal survival [RGD, Feb 2006]

Uniprot Description

SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protease binding; receptor binding

Biological Process: positive regulation of blood coagulation; wound healing; response to lipopolysaccharide; positive regulation of coagulation; decidualization; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; glucose homeostasis; mast cell activation; positive regulation of receptor-mediated endocytosis; positive regulation of interleukin-8 production; positive regulation of fibroblast proliferation; negative regulation of fibrinolysis; tissue regeneration; regulation of receptor activity; angiogenesis; chronological cell aging; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; female gonad development; positive regulation of keratinocyte migration; negative regulation of proteolysis; regulation of angiogenesis; regulation of cell proliferation; response to starvation; response to reactive oxygen species; positive regulation of angiogenesis; response to hyperoxia; cellular response to insulin stimulus; response to estrogen stimulus; response to cytokine stimulus; positive regulation of inflammatory response