Thrombopoietin Active Protein | mTPO active protein

Recombinant Mouse Thrombopoietin

Cat. Num. AAA142223

• Gene Names: Thpo; Ml; Tpo; Mgdf; Mpllg
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Thrombopoietin; Recombinant Mouse Thrombopoietin; TPO Mouse; Thrombopoietin Mouse Recombinant; Megakaryocyte colony-stimulating factor; Myeloproliferative leukemia virus oncogene ligand; C-mpl ligand; ML; Megakaryocyte growth and development factor; MGDF; TPO; MKCSF; MPLLG; MGC163194; THPO; mTPO active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF
• Sequence Length: 356

• Solubility: It is recommended to reconstitute the lyophilized Thrombopoietin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: ED50 range is less than 0.8 ng/ml as determined by the dose dependent stimulation of Mo7e cells.
• Preparation and Storage: Lyophilized Thrombopoietin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution TPO Mouse should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for mTPO active protein

Description: Thrombopoietin Mouse Recombinant produced in E Coli is a single, non-glycosylated soluble polypeptide chain containing 174 amino acids and having a molecular mass of 18704 Dalton. The TPO is purified by proprietary chromatographic techniques.

Introduction: Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidneyt hat regulates the production of platelets by the bone marrow. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that fragment into large numbers of platelets.

Product Categories/Family for mTPO active protein

Cytokines and Growth Factors; Cytokines; Thrombopoietin

NCBI and Uniprot Product Information

• NCBI GI #: 291045237
• NCBI GeneID: 21832
• NCBI Accession #: NP_001166976.1
• NCBI GenBank Nucleotide #: NM_001173505.1
• UniProt Accession #: P40226; JC4227
• Molecular Weight: 37,384 Da
• NCBI Official Full Name: thrombopoietin isoform 1
• NCBI Official Synonym Full Names: thrombopoietin
• NCBI Official Symbol: Thpo
• NCBI Official Synonym Symbols: Ml; Tpo; Mgdf; Mpllg
• NCBI Protein Information: thrombopoietin; C-mpl ligand; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; myeloproliferative leukemia virus oncogene ligand
• UniProt Protein Name: Thrombopoietin
• Protein Family: Thrombopoietin
• UniProt Gene Name: Thpo
• UniProt Synonym Gene Names: ML; MGDF
• UniProt Entry Name: TPO_MOUSE

NCBI Description

This gene encodes a humoral growth factor necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. The encoded protein is a ligand for the product of the myeloproliferative leukemia virus oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted; Cell cycle regulation; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: hormone activity; cytokine activity; receptor binding

Biological Process: positive regulation of protein kinase B signaling cascade; cell proliferation; myeloid cell differentiation; positive regulation of megakaryocyte differentiation; positive regulation of protein amino acid phosphorylation