Arginine Methyltransferase 1 Active Protein | mPRMT1 active protein

Recombinant Mouse Protein Arginine Methyltransferase 1

Cat. Num. AAA143377

• Gene Names: Prmt1; Mrmt1; Hrmt1l2; AW214366; 6720434D09Rik
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Arginine Methyltransferase 1; Recombinant Mouse Protein Arginine Methyltransferase 1; PRMT1 Mouse; Protein Arginine Methyltransferase 1 Mouse Recombinant; ANM1; HCP1; HRMT1L2; IR1B4; Interferon receptor 1-bound protein 4; EC 2.1.1; Protein arginine N-methyltransferase 1; Prmt1; AW214366; 6720434D09Rik; mPRMT1 active protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The PRMT1 solution (1mg/ml) contains 40mM Tris-HCl pH 8.0, 100mM NaCl, 4mM MgCl2, 2mM DTT and 40% glycerol.; Sterile Filtered colorless solution.
• Sequence: MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGILCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLHAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYVHALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR
• Sequence Length: 353

• Unit Defenition: One unit will transfer 1pmol of methyl group to synthetic peptide of histone H4 for 10 minutes at 37 degree C.
• Specific Activity: 10,000 Units/ml.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks.Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for mPRMT1 active protein

Description: PRMT1 Mouse Recombinant fused with His-MBP tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 750 amino acids and having a molecular mass of 84 kDa.The PRMT1 is purified by proprietary chromatographic techniques.

Introduction: PRMT1 Methylates (mono & asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in a glycine and arginine-rich domain (may methylate HNRNPA1 and histones). Methylates SUPT5H.The PRMT1 protein functions as a histone methyltransferase specific for H4.PRMT1 is an essential factor in oncogenesis and is a potential novel therapeutic target in cancer.PRMT1-mediated methylation serves as a positive modulator of IR/IRS-1/PI3K pathway and glucose uptake in skeletal muscle cells. CAF1 is a new regulator of PRMT1-dependent arginine methylation.PRMT1 arginine-methylates MRE11 therefore it regulates the activity of MRE11-RAD50-NBS1 complex during the intra-S-phase DNA damage checkpoint response.PRMT1 plays a post-translationally part in regulating the transcriptional activity.PRMT1is found predominantly in the cytoplasma though a fraction of PRMT1 is located in the nucleus.

Product Categories/Family for mPRMT1 active protein

Enzymes; Transferase

NCBI and Uniprot Product Information

• NCBI GI #: 357197158
• NCBI GeneID: 15469
• NCBI Accession #: NP_001239405.1
• NCBI GenBank Nucleotide #: NM_001252476.1
• UniProt Accession #: Q9JIF0; Q99LS4
• Molecular Weight: 39,576 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 1 isoform 2
• NCBI Official Synonym Full Names: protein arginine N-methyltransferase 1
• NCBI Official Symbol: Prmt1
• NCBI Official Synonym Symbols: Mrmt1; Hrmt1l2; AW214366; 6720434D09Rik
• NCBI Protein Information: protein arginine N-methyltransferase 1; heterogeneous nuclear ribonucleoproteins methyltransferase-like 2; histone-arginine N-methyltransferase PRMT1
• UniProt Protein Name: Protein arginine N-methyltransferase 1
• UniProt Gene Name: Prmt1
• UniProt Synonym Gene Names: Hrmt1l2; Mrmt1
• UniProt Entry Name: ANM1_MOUSE

Uniprot Description

PRMT1: Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Homodimer and heterodimer with PRMT8. The dimer can then associate to form a homohexamer. Interacts with ILF3, BTG1, BTG2, SUPT5H and interferon-alpha/beta receptor 1. Interacts with NFATC2IP. Widely expressed. By BTG1, BTG2 and ILF3. Belongs to the protein arginine N-methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.125; Methyltransferase; Methyltransferase, protein arginine; Nuclear receptor co-regulator

Cellular Component: protein complex; cytoplasm; cytosol; nucleus

Molecular Function: methyltransferase activity; identical protein binding; trimethylamine methyltransferase activity; monomethylamine methyltransferase activity; tRNA (cytosine)-methyltransferase activity; RNA methyltransferase activity; rRNA (cytosine-C5-967)-methyltransferase activity; rRNA (uridine) methyltransferase activity; snoRNP binding; N-methyltransferase activity; tRNA (guanine) methyltransferase activity; rRNA (adenine-N6,N6-)-dimethyltransferase activity; arginine N-methyltransferase activity; selenocysteine methyltransferase activity; methanol-specific methylcobalamin:coenzyme M methyltransferase activity; protein-arginine N-methyltransferase activity; tRNA (guanine-N2-)-methyltransferase activity; dimethylamine methyltransferase activity; [cytochrome c]-arginine N-methyltransferase activity; protein methyltransferase activity; 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity; hydroxyneurosporene-O-methyltransferase activity; transferase activity; rRNA (uridine-2'-O-)-methyltransferase activity; tRNA (uracil) methyltransferase activity; rRNA (adenine) methyltransferase activity; C-methyltransferase activity; cobalt-precorrin-6B C5-methyltransferase activity; cobalt-precorrin-3 C17-methyltransferase activity; 1-phenanthrol methyltransferase activity; S-methyltransferase activity; lysine N-methyltransferase activity; DNA-methyltransferase activity; protein-arginine omega-N monomethyltransferase activity; tRNA (cytosine-5-)-methyltransferase activity; C-terminal protein carboxyl methyltransferase activity; tRNA (adenine)-methyltransferase activity; rRNA (cytosine) methyltransferase activity; cobalt-precorrin-7 C15-methyltransferase activity; protein-arginine N5-methyltransferase activity; rRNA methyltransferase activity; histone methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; protein-leucine O-methyltransferase activity; histone-arginine N-methyltransferase activity; demethylmenaquinone methyltransferase activity; rRNA (adenine-N6-)-methyltransferase activity; tRNA methyltransferase activity; rRNA (guanine) methyltransferase activity; methylarsonite methyltransferase activity; mRNA methyltransferase activity; protein binding; methylamine-specific methylcobalamin:coenzyme M methyltransferase activity; tRNA (guanine-N1-)-methyltransferase activity; cobalt-precorrin-5B C1-methyltransferase activity; protein-lysine N-methyltransferase activity; O-methyltransferase activity; tRNA (adenine-57, 58-N(1)-) methyltransferase activity; tRNA (adenine-N1-)-methyltransferase activity

Biological Process: methylation; histone methylation; peptidyl-arginine methylation; regulation of transcription, DNA-dependent; protein amino acid methylation; in utero embryonic development; peptidyl-arginine omega-N-methylation; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; peptidyl-arginine N-methylation; neurite development; negative regulation of megakaryocyte differentiation