Betacellulin Active Protein | Btc active protein

Mouse Betacellulin

Cat. Num. AAA691628

• Gene Names: Btc; Bcn
• Reactivity: Mouse
• Purity: >98% by SDS-PAGE & HPLC analyses
• Synonyms: Betacellulin; Mouse Betacellulin; Recombinant Mouse Betacellulin; Btc active protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: >98% by SDS-PAGE & HPLC analyses
• Form/Format: Lyophilized
• Sequence: DGNTTRTPET NGSLCGAPGE NCTGTTPRQK VKTHFSRCPK QYKHYC IHGR CRFVVDEQTP SCICEKGYFG ARCERVDLFY
• Sequence Length: 80

• Endotoxin Level: < 0.1 ng per ug of Betacellulin
• Biological Activity: Determined by its ability to stimulate the proliferation of mouse Balb/3T3 cells. The expected ED50 is <= 0.01 ng/ml, corresponding to a specific activity of >= 1 x 10^8 units/mg.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Reconstituted Betacellulin is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 degree C.

Related Product Information for Btc active protein

Betacellulin (BTC or beta-TC) is a member of the EGF family of cytokines which includes EGF, TGFalpha, amphiregulin, HB-EGF, epiregulin, tomoregulin and the neuregulins. All EGF famiy members are synthesized as type I transmembrane precursor proteins containing one or more EGF-like domains in their extracellular region. BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotien. The mouse BTC cDNA encodes a 177 amino acid (aa) residue precursor with a 31 aa signal peptide, an 87 aa residue extracellular region containing one EGF-like domain, a 21 aa transmembrane domain and a 38 aa cytoplasmic domain. Soluble BTC is released from the transmembrane precursor by proteolytic processing. Mouse BTC shares 93% and 79% aa sequence identity with rat and human BTC, respectively. The mouse BTC gene is tightly linked to that of amphiregulin on mouse chromosome 5. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids including serum, milk and colostrum. BTC promotes pancreatic betacell growth and differentiation and is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts. The effects of BTC is mediated by binding to ErbB1 and ErbB4 homodimers as well as ErbB heterodimers. Recombinant murine Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

Product Categories/Family for Btc active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 6680812
• NCBI GeneID: 12223
• NCBI Accession #: NP_031594.1
• NCBI GenBank Nucleotide #: NM_007568.5
• UniProt Accession #: Q05928
• Molecular Weight: 9.0 kDa
• NCBI Official Full Name: betacellulin preproprotein
• NCBI Official Synonym Full Names: betacellulin, epidermal growth factor family member
• NCBI Official Symbol: Btc
• NCBI Official Synonym Symbols: Bcn
• NCBI Protein Information: betacellulin; probetacellulin
• UniProt Protein Name: Probetacellulin
• Protein Family: Probetacellulin
• UniProt Gene Name: Btc
• UniProt Synonym Gene Names: Bcn; BTC
• UniProt Entry Name: BTC_MOUSE

NCBI Description

This gene encodes a member of the epidermal growth factor (EGF) family. These growth factors are ligands for the EGFR/ErbB receptor tyrosine kinases, and play roles in cell growth and differentiation. The encoded protein is synthesized as a transmembrane precursor that is proteolytically cleaved to generate a mature peptide, and plays a role in the differentiation of pancreatic beta cells. This gene may also play a protective role in acute pancreatitis, whereas increased expression of this gene may contribute to diabetic macular edema. Gene therapy using combinations of this gene and other pancreas-specific transcription factors may induce islet neogenesis and remediate hyperglycemia in type 1 diabetes. [provided by RefSeq, Apr 2011]

Uniprot Description

Function: Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Ref.3

Subunit structure: Monomer. Interacts with EGFR and ERBB4.

Subcellular location: Betacellulin: Secreted › extracellular space. Probetacellulin: Cell membrane; Single-pass type I membrane protein.

Tissue specificity: Found in several mouse tissues including kidney, uterus and liver, as well as in beta tumor cell line and MCF-7 cells. It is not detected in the brain.

Sequence similarities: Contains 1 EGF-like domain.