Macrophage Inflammatory protein-3 beta Active Protein | MIP 3b active protein

Recombinant Human Macrophage Inflammatory protein-3 beta (CCL19)

Cat. Num. AAA142015

• Gene Names: CCL19; ELC; CKb11; MIP3B; MIP-3b; SCYA19
• Purity: Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Macrophage Inflammatory protein-3 beta; Recombinant Human Macrophage Inflammatory protein-3 beta (CCL19); MIP 3b Human; Macrophage Inflammatory Protein-3 Beta Human Recombinant (CCL19); Small inducible cytokine A19; CCL19; Macrophage inflammatory protein 3 beta; MIP-3- beta; EBI1-ligand chemokine; ELC; Beta chemokine exodus-3; CK beta-11; chemokine (C-C motif) ligand 19; CKb11; MIP3B; MIP-3b; SCYA19; MGC34433; MIP 3b active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Lyophilized without any additives.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS
• Sequence Length: 98

• Solubility: It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-3b in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The Activity of CCL19 is calculated by the ability to chemoattract Human T cells using a concentration of 7-40ng/ml corresponding to a Specific Activity of 25,000-143,000IU/mg.
• Preparation and Storage: Lyophilized MIP-3b although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CCL19 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for MIP 3b active protein

Description: Macrophage Inflammatory Protein-3 beta Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8.8kDa. The MIP-3b is purified by proprietary chromatographic techniques.

Introduction: Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). CCL19 is expressed abundantly in thymus and lymph nodes, with moderate levels in trachea and colon and low levels in stomach, small intestine, lung, kidney and spleen. The gene for CCL19 is located on human chromosome 9. This chemokine elicits its effects on its target cells by binding to the chemokine receptor chemokine receptor CCR7. It attracts certain cells of the immune system, including dendritic cells and antigen-engaged B cells.

Product Categories/Family for MIP 3b active protein

Chemokines; MIP

NCBI and Uniprot Product Information

• NCBI GI #: 5454040
• NCBI GeneID: 6363
• NCBI Accession #: NP_006265.1
• NCBI GenBank Nucleotide #: NM_006274.2
• UniProt Accession #: Q99731; O00697; O00736
• Molecular Weight: 10,993 Da
• NCBI Official Full Name: C-C motif chemokine 19
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 19
• NCBI Official Symbol: CCL19
• NCBI Official Synonym Symbols: ELC; CKb11; MIP3B; MIP-3b; SCYA19
• NCBI Protein Information: C-C motif chemokine 19; CC chemokine ligand 19; CK beta-11; EBI1 ligand chemokine; EBI1-ligand chemokine; MIP-3-beta; beta chemokine exodus-3; beta-chemokine exodus-3; epstein-Barr virus-induced molecule 1 ligand chemokine; macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; small inducible cytokine subfamily A (Cys-Cys), member 19; small-inducible cytokine A19
• UniProt Protein Name: C-C motif chemokine 19
• UniProt Gene Name: CCL19
• UniProt Synonym Gene Names: ELC; MIP3B; SCYA19; EBI1 ligand chemokine; ELC; MIP-3-beta
• UniProt Entry Name: CCL19_HUMAN

NCBI Description

This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL19: May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: extracellular space; extracellular region

Molecular Function: CCR chemokine receptor binding; chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding; CCR7 chemokine receptor binding

Biological Process: formation of immunological synapse; positive regulation of interleukin-12 production; positive regulation of dendritic cell antigen processing and presentation; cell maturation; positive regulation of JNK cascade; positive regulation of NF-kappaB import into nucleus; positive regulation of endocytosis; positive regulation of interleukin-1 beta secretion; activation of JNK activity; positive regulation of receptor-mediated endocytosis; dendritic cell chemotaxis; establishment of T cell polarity; positive regulation of T cell proliferation; cell communication; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of chemotaxis; response to virus; positive regulation of phosphoinositide 3-kinase activity; positive regulation of tumor necrosis factor production; cellular calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; myeloid dendritic cell chemotaxis; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; T cell costimulation; immune response; positive regulation of T-helper 1 cell differentiation