Macrophage Inflammatory protein-3 Active Protein | MIP 3 active protein

Recombinant Human Macrophage Inflammatory protein-3 (CCL23)

Cat. Num. AAA143488

• Gene Names: CCL23; CKb8; MIP3; Ckb-8; MIP-3; MPIF-1; SCYA23; Ckb-8-1; hmrp-2a; CK-BETA-8
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Macrophage Inflammatory protein-3; Recombinant Human Macrophage Inflammatory protein-3 (CCL23); MIP 3 Human; Macrophage Inflammatory Protein-3 Human Recombinant (CCL23); C-C motif chemokine 23; Small-inducible cytokine A23; Macrophage inflammatory protein 3; Myeloid progenitor inhibitory factor 1; CK-beta-8; MIP-3; MPIF-1; CKB-8; CCL23; MIP3; MPIF1; SCYA23; CKb8; Ckb-8-1; MIP 3 active protein

• Host: E Coli
• Purity/Purification: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Filtered (0.2 um) and lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESYFETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN
• Sequence Length: 137

• Solubility: It is recommended to reconstitute the lyophilized MIP-3 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Determined by its ability to chemoattract human T cell population using a concentration range of 10-50ng/ml corresponding to a Specific Activity of 20,000-100,000IU/mg.
• Preparation and Storage: Lyophilized MIP-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CCL23 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for MIP 3 active protein

Description: MIP-3 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 99 amino acids and having a molecular mass of 11.3kDa. The MIP-3 is purified by proprietary chromatographic techniques.

Introduction: CCL23 (MIP-3) is a ligand for the CCR1chemokine receptor. CCL23 is one of several cytokine genes clustered on the q-arm of chromosome 17, in a locus containing several other CC chemokines. MIP-3 chemoattracts monocytes, resting T-lymphocytes and neutrophils, but not activated lymphocytes. Furthermore, it was shown that MIP-3 inhibits colony formation of bone marrow myeloid immature progenitors. MIP-3 is mainly expressed in lung and liver tissue, but can be also found in bone marrow and placenta, as well as in some cell lines of myeloid origin. Alternative splicing of the CCL23 gene produces 2 mRNAs which encode a short (CK?8) and a long (CK?81) isoform of the MIP-3. CK?8 cDNA encodes a 120 amino acid residue precursor protein with a putative 21 a.a. residue signal peptide which is cleaved to generate a 99 a.a. residue mature CK?8 (a.a. 22-120). Further N-terminal processing of the 99 a.a. residue variant can produce a 75 a.a. residue CK?8 (a.a. 46-120) which is considerably more active than the 99 a.a. residue variant.MIP-3 may be involved in the malignant progression of certain human cancer cells which overexpress ErbB2 through the transactivation of ErbB2 tyrosine kinase. MIP-3 may also be involved in angiogenesis via upregulation of matrix metalloproteinase MMP-2 expression.

Product Categories/Family for MIP 3 active protein

Chemokines; MIP

NCBI and Uniprot Product Information

• NCBI GI #: 632794829
• NCBI GeneID: 6368
• NCBI Accession #: NP_005055.3
• NCBI GenBank Nucleotide #: NM_005064.5
• UniProt Accession #: P55773; O00174; O75950; Q52LD4; B7ZKQ3
• Molecular Weight: 15,290 Da
• NCBI Official Full Name: C-C motif chemokine 23 isoform CKbeta8-1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 23
• NCBI Official Symbol: CCL23
• NCBI Official Synonym Symbols: CKb8; MIP3; Ckb-8; MIP-3; MPIF-1; SCYA23; Ckb-8-1; hmrp-2a; CK-BETA-8
• NCBI Protein Information: C-C motif chemokine 23; C6 beta-chemokine; macrophage inflammatory protein 3; myeloid progenitor inhibitory factor 1; small inducible cytokine subfamily A (Cys-Cys), member 23
• UniProt Protein Name: C-C motif chemokine 23
• UniProt Gene Name: CCL23
• UniProt Synonym Gene Names: MIP3; MPIF1; SCYA23; CKB-8; MIP-3; MPIF-1
• UniProt Entry Name: CCL23_HUMAN

NCBI Description

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils and no activity on activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. In addition, the product of this gene is a potent agonist of the chemokine (C-C motif) receptor 1. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

CCL6: Shows chemotactic activity for monocytes, resting T- lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CCR1 chemokine receptor binding; chemokine activity

Biological Process: cellular calcium ion homeostasis; monocyte chemotaxis; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; cell-cell signaling; immune response; chemotaxis; inflammatory response; signal transduction