interleukin 1 alpha Active Protein | mIL-1a active protein

Mouse interleukin 1 alpha

Cat. Num. AAA400091

• Gene Names: IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA
• Purity: Greater than 98.0% as determined by:; (a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.; (b) Gel filtration chromatography under non-denaturing conditions.
• Synonyms: interleukin 1 alpha; Mouse interleukin 1 alpha; Mouse Interleukin 1 alpha (mIL-1a); mIL-1a active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by:; (a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.; (b) Gel filtration chromatography under non-denaturing conditions.
• Form/Format: Lyophylized white powder
• Sequence: The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ser-Ala-Pro-Tyr-Thr.
• Sequence Length: 271

• Solubility: It is recommended to reconstitute the lyophilized IL-1a in sterile 18Momega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions containing carrier protein such as BSA, HSA or similar.
• Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 1.33 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the DNAman computer analysis program of protein sequences.
• Dimers and Aggregates: Less than 2% as determined by gel filtration under non-denaturing conditions.
• Endotoxin: Less than 0.05 ng/ug (0.5 EU/ug) of recombinant IL-1a.
• Formulation: Interleukin-1a is lyophilized from a concentrated (1mg/ml) solution in water containing NaHCO3.
• Biological Activity: murine IL-1a is biologically active in helper T cell line D10.G4.1
• Preparation and Storage: Lyophilized IL-1-alpha although stable at room temperature for 3 weeks, should be stored desiccated below -180 degree C. Upon reconstitution Interleukin-3 should be stored at 40C between 2-7 days and for future use below -180 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Please avoid freeze-thaw cycles.

Related Product Information for mIL-1a active protein

IL-1a is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1a and IL-1b binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. Recombinant mouse IL-1a is an 18.4 kDa protein containing 157 amino acid residues. Recombinant monomeric murine IL-1a is purified by proprietary chromatographic techniques.

Product Categories/Family for mIL-1a active protein

Hormones; Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 27894330
• NCBI GeneID: 3552
• NCBI Accession #: NP_000566.3
• NCBI GenBank Nucleotide #: NM_000575.3
• UniProt Accession #: P01583; Q53QF9; Q7RU02
• Molecular Weight: 30,607 Da
• NCBI Official Full Name: interleukin-1 alpha proprotein
• NCBI Official Synonym Full Names: interleukin 1, alpha
• NCBI Official Symbol: IL1A
• NCBI Official Synonym Symbols: IL1; IL-1A; IL1F1; IL1-ALPHA
• NCBI Protein Information: interleukin-1 alpha; IL-1 alpha; hematopoietin-1; preinterleukin 1 alpha; pro-interleukin-1-alpha
• UniProt Protein Name: Interleukin-1 alpha
• UniProt Gene Name: IL1A
• UniProt Synonym Gene Names: IL1F1; IL-1 alpha
• UniProt Entry Name: IL1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family.

Protein type: Motility/polarity/chemotaxis; Cytokine

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein binding; interleukin-1 receptor binding; copper ion binding; cytokine activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; apoptosis; positive regulation of interleukin-2 biosynthetic process; cytokine and chemokine mediated signaling pathway; germ cell programmed cell death; positive regulation of JNK cascade; fever; cell proliferation; negative regulation of cell proliferation; positive regulation of angiogenesis; connective tissue replacement during inflammatory response; response to copper ion; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response; positive regulation of cytokine secretion