Leptin Active Protein | LPT active protein

Human Leptin Protein

Cat. Num. AAA9718732

• Gene Names: LEP; OB; OBS; LEPD
• Purity: >98% as determined by SDS-PAGE
• Synonyms: Leptin; Human Leptin Protein; Recombinant Human Leptin Protein (Leptin) expressed in E. coli; LEPD; OB; OBS; LPT active protein

• Host: E Coli
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence: Amino acid sequence derived from mature form of human Leptin (NP_000221.1) (Val 22-Cys 167) was expressed, with an additional Met.
• Sequence Length: 167

• Species: Human
• Protein Length: The recombinant human leptin consists of 147 amino acids and predicts a molecular mass of 16 kDa. The apparent molecular mass of rhLEP is approximately 13 kDa in SDS-PAGE under reducing conditions.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human Leptin at 1.25 mug/ml(100 mul/well) can bind human Leptin receptor Fc chimera with a linear range of 0.032-4.0 mug/ml. Immobilized human Leptin at 5 mug/ml(100 mul/well) can bind human Leptin receptor his with a linear range of 0.032-4.0 mug/ml. 1. Immobilized human Leptin at 1.25 mug/ml (100 mul/well) can bind human Leptin receptor Fc chimera with a linear range of 0.032-4.0 mug/ml. 2. Immobilized human Leptin at 5 mug/ml (100 mul/well) can bind human Leptin receptor his with a linear range of 0.032-4.0 mug/ml.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human Leptin protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human Leptin protein.)

Related Product Information for LPT active protein

Leptin is one of the most important hormones secreted by adipocytes, as an adipokine that modulates multiple functions including energy homeostasis, thermoregulation, bone metabolism, endocrine and pro-inflammatory immune responses. The circulating leptin levels serve as a gauge of energy stores, thereby directing the regulation of energy homeostasis, neuroendocrine function, and metabolism. Recent studies suggest that leptin is physiologically more important as an indicator of energy deficiency, rather than energy excess, and may mediate adaptation by driving increased food intake and directing neuroendocrine function to converse energy, such as inducing hypothalamic hypogonadism to prevent fertilization. One of these functions is the connection between nutritional status and immune competence. The adipocyte-derived hormone Leptin has been shown to regulate the immune response, innate and adaptive response, both in normal and pathological conditions. Thus, Leptin is a mediator of the inflammatory response. Leptin has a dual effect on bone, acting by two independent mechanisms. As a signal molecule with growth factor characteristics, leptin is able to stimulate osteoblastic cells and to inhibit osteoclast formation and activity, thus promoting osteogenesis. However, as a molecule which stimulates sympathetic neurons in the hypothalamus, leptin indirectly inhibits bone formation. This inhibitory effect of leptin mediated by activation of sympathetic nervous system can be abrogated by application of blood pressure-reducing beta-blockers, which also inhibit receptors of hypothalamic adrenergic neurons. Leptin appears to regulate a number of features defining Alzheimer's disease (AD) at the molecular and physiological level. Leptin can stimulate mitogenic and angiogenic processes in peripheral organs. Because leptin levels are elevated in obese individuals and excess body weight has been shown to increase breast cancer risk in postmenopausal women. Furthermore, a recent report clearly shows that targeting leptin signaling may reduce mammary carcinogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 4557715
• NCBI GeneID: 3952
• NCBI Accession #: NP_000221.1
• NCBI GenBank Nucleotide #: NP_000221.1
• UniProt Accession #: P41159
• Molecular Weight: 16 kDa
• NCBI Official Full Name: leptin
• NCBI Official Synonym Full Names: leptin
• NCBI Official Symbol: LEP
• NCBI Official Synonym Symbols: OB; OBS; LEPD
• NCBI Protein Information: leptin
• Protein Family: Leptin

NCBI Description

This gene encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Aug 2017]