Galectin-3 Active Protein | LGALS3 active protein

Recombinant Human Galectin-3

Cat. Num. AAA143304

• Gene Names: LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• Purity: Greater than 97.0% as determined by analysis by SDS-PAGE and HPLC.
• Synonyms: Galectin-3; Recombinant Human Galectin-3; LGALS3 Human; Galectin-3 Human Recombinant; GAL3; MAC2; CBP35; GALB; GALIG; LGALS2; LGALS3; Galactose-specific lectin 3; Mac-2 antigen; IgE-binding protein; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Laminin-binding protein; Lectin L-29; L-31; Galactoside-binding protein; GALBP; LGALS3 active protein

• Host: E Coli
• Purity/Purification: Greater than 97.0% as determined by analysis by SDS-PAGE and HPLC.
• Form/Format: The Galectin-3 protein was lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4 and 3mM DTT.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYPGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSATGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI
• Sequence Length: 250

• Solubility: It is recommended to reconstitute the lyophilized LGALS3 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED50 as determined by its ability to agglutinate human red blood cells is less than 10 ug/ml.
• Preparation and Storage: Lyophilized Galectin-3 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Galectin-3 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for LGALS3 active protein

Description: LGALS3 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 249 amino acids and having a molecular mass of 26kDa.The LGALS3 is purified by proprietary chromatographic techniques.

Introduction: Galectin-3 mediates with the alpha-3, beta-1 integrin the stimulation by cspg4 of endothelial cells migration. Galectin-3 plays an necessary part during the acquisition of vasculogenic mimicry and angiogenic properties associated with melanoma progression. LGALS3 overexpression is highly expressed in early stages of papillary carcinoma, and its expression intensity declines during tumor progression. Serum levels of LGALS3 are high in patients with thyroid malignancy but there is considerable overlap in serum LGALS3 concentrations between those with benign and malignant nodular thyroid disease. LGLAS3 takes part as an immune regulator to inhibit T-cell immune responses and promote tumor growth, as a result providing a new mechanism for tumor immune tolerance.

Product Categories/Family for LGALS3 active protein

Cytokines and Growth Factors; Growth Factors; Galectin

NCBI and Uniprot Product Information

• NCBI GI #: 115430223
• NCBI GeneID: 3958
• NCBI Accession #: NP_002297.2
• NCBI GenBank Nucleotide #: NM_002306.3
• UniProt Accession #: P17931; Q16005; Q6IBA7; Q96J47; B2RC38
• Molecular Weight: 26,152 Da
• NCBI Official Full Name: galectin-3 isoform 1
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 3
• NCBI Official Symbol: LGALS3
• NCBI Official Synonym Symbols: L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• NCBI Protein Information: galectin-3; 35 kDa lectin; IgE-binding protein; MAC-2 antigen; carbohydrate-binding protein 35; galactose-specific lectin 3; laminin-binding protein; lectin L-29
• UniProt Protein Name: Galectin-3
• Protein Family: Galectin
• UniProt Gene Name: LGALS3
• UniProt Synonym Gene Names: MAC2; Gal-3; CBP 35; GALBP
• UniProt Entry Name: LEG3_HUMAN

NCBI Description

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Uniprot Description

Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.

Protein type: Motility/polarity/chemotaxis; Extracellular matrix; Cell surface

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: spliceosome; extracellular matrix; extracellular space; proteinaceous extracellular matrix; membrane; mitochondrial inner membrane; cytoplasm; plasma membrane; immunological synapse; nucleus; external side of plasma membrane

Molecular Function: protein binding; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; negative regulation of endocytosis; RNA splicing; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; macrophage chemotaxis; monocyte chemotaxis; epithelial cell differentiation; positive chemotaxis; eosinophil chemotaxis; innate immune response; mRNA processing; skeletal development