Interleukin-18 Active Protein | IL-18 active protein

Human Interleukin-18 Recombinant

Cat. Num. AAA553113

• Purity: >92%, as determined by SDS-PAGE and HPLC
• Synonyms: Interleukin-18; Human Interleukin-18 Recombinant; IGIF; IL1F4; IL18; il-18; IL-18 active protein

• Host: E Coli
• Purity/Purification: >92%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Interleukin-18 was lyophilized from 0.2 um filtered PBS solution pH 7.4.
• Sequence: MAAEPVEDNC INFVAMKFID NTLYFIAEDD ENLESDYFGK LESKLSVIRN LNDQVLFIDQ GNRPLFEDMT DSDCRDNAPR TIFIISMYKD SQPRGMAVTI SVKCEKISTL SCENKIISFK EMNPPDNIKD TKSDIIFFQR SVPGHDNKMQ FESSSYEGYF LACEKERDLF KLILKKEDEL GDRSIMFTVQ NED
• Sequence Length: 189

• Domain: IL1
• Host Note: Optimized DNA sequence encoding Human Interleukin-18 mature chain was expressed in Escherichia Coli
• Biological Activity: The ED(50) was determined by the dose-dependent proliferation of human PBMC co-stimulated with IL-12 cells was found to be in the range of 5ng/ml.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Molecular Weight Note: Native human Interleukin-18, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 18 kDa. Recombinant Interleukin-18 is a disulfide-linked monomer protein consisting of 158 amino acid residue subunits, migrates as an approximately 18 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein in stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detecable loss of activity.; Avoid repeated freeze/thaw cycles.

Related Product Information for IL-18 active protein

IL18 is synthesized as a biologically inactive precursor protein that has limited biological activity. The proform of IL18 is processed by one of the caspases, Caspase-1, to generate the 18 kDa bioactive molecule. IL-18 is produced during the acute immune response by macrophages and immature dendritic cells. Interleukin-18 is expressed by a variety of immune and non-immune cells, including monocytes and macrophages. IL18 is one of the pro- inflammatory cytokines. The activities of IL18 appear to be species specific. An important function of IL18 is the regulation of functionally distinct subsets of T-helper cells required for cell mediated immune responses (Nakanishi et al, 2001). IL18 functions as a growth and differentiation factor for Th1 cells. IL18 up-regulates FAS ligand mediated cytotoxic activitiy of murine natural killer cells. IL-18 is part of a complex regulatory circuit involved in causing cell death by apoptosis.

Product Categories/Family for IL-18 active protein

Cytokines

References

• IL-18 binding protein-expressing mesenchymal stem cells improve myocardial protection after ischemia or infarction .PNAS, Sep 2009; 10.1073/pnas.0908924106.
• IL-18 and urinary ngal predict dialysis and graft recovery after kidney transplantation .J. Am. Soc. Nephrol., Sep 2009; 10.1681/ASN.2009030264.
• a functional polymorphism in IL-18 is associated with severity of bronchial asthma .Am. J. Respir. Crit. Care Med., Sep 2009; 10.1164/rccm.200905-0652 degree C.
• il-12p40 and IL-18 play pivotal roles in orchestrating the cell-mediated immune response to a poxvirus infection .J. Immunol., Sep 2009; 183: 3324 - 3331.
• neutrophil chemokines and their role in IL-18-mediated increase in neutrophil o2– production and intestinal edema following alcohol intoxication and burn injury .Am J Physiol Gastrointest Liver Physiol, Aug 2009; 297: G340 - G347.