Interferon-gamma Active Protein | IFNG active protein

Recombinant Human Interferon-gamma

Cat. Num. AAA142083

• Gene Names: IFNG; IFG; IFI
• Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Interferon-gamma; Recombinant Human Interferon-gamma; IFN g Human; Interferon-Gamma Human Recombinant; Immune Interferon; type II interferon; T cell interferon; MAF; IFNG; IFG; IFI; IFN-gamma; IFNG active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: The protein was lyophilized from a 0.2 um filtered concentrated solution in PBS pH 4.5.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQIVSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYSVTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ
• Sequence Length: 166

• Solubility: It is recommended to reconstitute the lyophilized Interferon-gamma in sterile distilled water or 20mM AcOH not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The specific activity as determined in a viral resistance assay is < 0.05 ng/ml, corresponding to a specific activity of 2.0 x 10,000,000 IU/mg.
• Preparation and Storage: Lyophilized Interferon gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IFN-gamma should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for IFNG active protein

Description: Interferon-gamma Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 144 amino acids and having a molecular mass of 17kDa.The IFN-gamma is purified by proprietary chromatographic techniques.

Introduction: IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Product Categories/Family for IFNG active protein

Cytokines and Growth Factors; Cytokines; Interferon

NCBI and Uniprot Product Information

• NCBI GI #: 56786138
• NCBI GeneID: 3458
• NCBI Accession #: NP_000610.2
• NCBI GenBank Nucleotide #: NM_000619.2
• UniProt Accession #: P01579; Q53ZV4; B5BU88
• Molecular Weight: 19,348 Da
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon, gamma
• NCBI Official Symbol: IFNG
• NCBI Official Synonym Symbols: IFG; IFI
• NCBI Protein Information: interferon gamma; IFN-gamma; immune interferon
• UniProt Protein Name: Interferon gamma
• Protein Family: Interferon
• UniProt Gene Name: IFNG
• UniProt Synonym Gene Names: IFN-gamma
• UniProt Entry Name: IFNG_HUMAN

NCBI Description

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]

Uniprot Description

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Cytokine; Membrane protein, integral; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: interferon-gamma receptor binding; cytokine activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of interleukin-23 production; positive regulation of interleukin-12 production; negative regulation of smooth muscle cell proliferation; apoptosis; positive regulation of osteoclast differentiation; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of interleukin-1 beta secretion; sensory perception of mechanical stimulus; negative regulation of transcription from RNA polymerase II promoter; positive regulation of killing of cells of another organism; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; neutrophil chemotaxis; positive regulation of synaptic transmission, cholinergic; adaptive immune response; negative regulation of myelination; CD8-positive, alpha-beta T cell differentiation during immune response; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; positive regulation of tumor necrosis factor production; defense response to protozoan; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell motility; regulation of insulin secretion

Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Tuberous Sclerosis 2; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To