Vimentin Active Protein | VIM active protein

Human Vimentin

Cat. Num. AAA691558

• Gene Names: VIM; HEL113; CTRCT30
• Reactivity: Human
• Purity: >95% by SDS-PAGE & HPLC analysis
• Synonyms: Vimentin; Human Vimentin; Vim; VIM active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: >95% by SDS-PAGE & HPLC analysis
• Form/Format: Lyophilized
• Sequence: STRSVSSSSY RRMFGGPGTA SRPSSSRSYV TTSTRTYSLG SALRPS TSRS LYASSPGGVY ATRSSAVRLR SSVPGVRLLQ DSVDFSLADA I NTEFKNTRT NEKVELQELN DRFANYIDKV RFLEQQNKIL LAELEQL KGQ GKSRLGDLYE EEMRELRRQV DQLTNDKARV EVERDNLAED IM RLREKLQE EMLQREEAEN TLQSFRQDVD NASLARLDLE RKVESLQE EI AFLKKLHEEE IQELQAQIQE QHVQIDVDVS KPDLTAALRD VRQ QYESVAA KNLQEAEEWY KSKFADLSEA ANRNNDALRQ AKQESTEYR R QVQSLTCEVD ALKGTNESLE RQMREMEENF AVEAANYQDT IGRL QDEIQN MKEEMARHLR EYQDLLNVKM ALDIEIATYR KLLEGEESRI SLPLPNFSSL NLRETNLDSL PLVDTHSKRT LLIKTVETRD GQVIN ETSQH HDDLEHHHHH H
• Sequence Length: 466

• Label/Conjugation: His-Tag
• Biological Activity: Data not available.

Related Product Information for VIM active protein

Vimentin is a class III intermediate filament protein predominantly found in cells of mesenchymal origins, such as vascular endothelium and blood cells, where it functions as a major cytoskeletal component. Due to its importance and abundance in the cytoskeletal structure of mesenchymally-derived cells, Vimentin is frequently used as a developmental marker within cells of mesenchymal origin or cells undergoing epithelial-mesenchymal transition, which can occur during both normal and metastatic growth. An active participant within several critical processes of cellular organization and protein regulation, Vimentin is involved in the anchorage of organelles within the cytoplasmic matrix, development of astrocytes, and the disassembly of cellular components during the execution phase of apoptosis. Abnormalities in the normal physiological pathways of Vimentin have been implicated in deficient motility and directional migration involved in wound healing and cellular growth and development, as well as the adhesion-site accumulation of Vimentin on lens epithelial cells in cases of dominant cataracts. Recombinant Human Vimentin is a 54.3 kDa protein consisting of 471 amino acid residues, including a 6 residue C-terminal His-Tag.

Product Categories/Family for VIM active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 62414289
• NCBI GeneID: 7431
• NCBI Accession #: NP_003371.2
• NCBI GenBank Nucleotide #: NM_003380.3
• UniProt Accession #: P08670; Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4
• Molecular Weight: 53,652 Da
• NCBI Official Full Name: vimentin
• NCBI Official Synonym Full Names: vimentin
• NCBI Official Symbol: VIM
• NCBI Official Synonym Symbols: HEL113; CTRCT30
• NCBI Protein Information: vimentin; epididymis luminal protein 113
• UniProt Protein Name: Vimentin
• Protein Family: Vimentin
• UniProt Gene Name: VIM
• UniProt Entry Name: VIME_HUMAN

NCBI Description

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Uniprot Description

Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Ref.38Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Ref.38

Subunit structure: Homopolymer assembled from elementary dimers. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain)

By similarity. Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B

By similarity. Ref.23 Ref.27 Ref.32 Ref.38 Ref.44

Subcellular location: Cytoplasm Ref.40.

Tissue specificity: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. Ref.12 Ref.17

Domain: The central alpha-helical coiled-coil rod region mediates elementary homodimerization. Ref.44

Post-translational modification: Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin

By similarity. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Ref.14 Ref.19 Ref.20 Ref.21 Ref.27 Ref.40O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. Ref.35

Involvement in disease: Cataract 30 (CTRCT30) [MIM:116300]: An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function.Note: The disease is caused by mutations affecting the gene represented in this entry.

Sequence similarities: Belongs to the intermediate filament family.

Sequence caution: The sequence BAB71275.1 differs from that shown. Reason: Intron retention.