G-CSF active protein

Human G-CSF

Cat. Num. AAA691630

• Gene Names: CSF3; GCSF; CSF3OS; C17orf33
• Reactivity: Human
• Purity: > 98% by SDS-PAGE and HPLC analyses
• Synonyms: G-CSF; Human G-CSF; Recombinant Human G-CSF; G-CSF active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 98% by SDS-PAGE and HPLC analyses
• Form/Format: Lyophilized
• Sequence: TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEE LVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ A LEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAM PAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP
• Sequence Length: 207

• Endotoxin Level: < 0.1 ng per g of G-CSF
• Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is <= 0.1 ng/ml, corresponding to a specific activity of >= 1 x 107 units/mg.

Related Product Information for G-CSF active protein

G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and mouse G-CSF are cross-species reactive. Recombinant human G-CSF is an 18.7 kDa protein consisting of 174 amino acid residues.

Product Categories/Family for G-CSF active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4503079
• NCBI GeneID: 1440
• NCBI Accession #: NP_000750.1
• NCBI GenBank Nucleotide #: NM_000759.3
• UniProt Accession #: P09919; A8MXR7
• Molecular Weight: 18.7 kDa
• NCBI Official Full Name: granulocyte colony-stimulating factor isoform a
• NCBI Official Synonym Full Names: colony stimulating factor 3 (granulocyte)
• NCBI Official Symbol: CSF3
• NCBI Official Synonym Symbols: GCSF; CSF3OS; C17orf33
• NCBI Protein Information: granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin
• UniProt Protein Name: Granulocyte colony-stimulating factor
• UniProt Gene Name: CSF3
• UniProt Synonym Gene Names: C17orf33; GCSF; G-CSF
• UniProt Entry Name: CSF3_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Post-translational modification: O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

Pharmaceutical use: Available under the names Neupogen or Granulokine (Amgen/Roche) and Granocyte (Rhone-Poulenc). Used to treat neutropenia (a disorder characterized by an extremely low number of neutrophils in blood).

Sequence similarities: Belongs to the IL-6 superfamily.

Caution: Ref.7 misquotes the gene name as "CSF1".