CYR61 active protein

Human CYR61

Cat. Num. AAA691555

• Gene Names: CYR61; CCN1; GIG1; IGFBP10
• Reactivity: Human
• Purity: > 95% by SDS-PAGE and HPLC analysis
• Synonyms: CYR61; Human CYR61; Recombinant Human CYR61; CYR61 active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 95% by SDS-PAGE and HPLC analysis
• Form/Format: Lyophilized from 10 mM Sodium Citrate, pH 3.0 + 50mM NaCl
• Sequence: MTCPAACHCP LEAPKCAPGV GLVRDGCGCC KVCAKQLNED CSKTQP CDHT KGLECNFGAS STALKGICRA QSEGRPCEYN SRIYQNGESF Q PNCKHQCTC IDGAVGCIPL CPQELSLPNL GCPNPRLVKV TGQCCEE WVC DEDSIKDPME DQDGLLGKEL GFDASEVELT RNNELIAVGK GS SLKRLPVF GMEPRILYNP LQGQKCIVQT TSWSQCSKTC GTGISTRV TN DNPECRLVKE TRICEVRPCG QPVYSSLKKG KKCSKTKKSP EPV RFTYAGC LSVKKYRPKY CGSCVDGRCC TPQLTRTVKM RFRCEDGET F SKNVMMIQSC KCNYNCPHAN EAAFPFYRLF NDIHKFRD
• Sequence Length: 381

• Endotoxin Level: < 0.1 ng per ug of CYR61
• Biological Activity: Determined by the dose-dependent stimulation of the proliferation of murine 3T3 cells. The expected ED50 for this effect is 2.0-3.0 ug/ml
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.
• Preparation and Storage: The lyophilized protein is stable at room temperature for up to 1 month. Working aliquots stored with a carrier protein are stable for at least 5 months at -20 degree C to -80 degree C. Avoid repeated freeze thaw cycles.

Related Product Information for CYR61 active protein

CYR61 is a member of the CCN family of secreted cysteine rich regulatory proteins. CYR61 induces angiogenesis by stimulating the proliferation, migration, and adhesion of endothelial cells. Cell migration and adhesion are mediated through binding to specific cell surface integrins and to heparin sulfate proteoglycans. Increased expression of CYR61 is associated with several types of cancer, and correlates with the progression and estrogen independence of human breast cancers. Recombinant human CYR61 is a 39.5 kDa protein containing 357 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the Thrombospondin type-I (TSP type-1) domain, and a C-terminal cysteine knot-like domain (CTCK).

Product Categories/Family for CYR61 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 31542331
• NCBI GeneID: 3491
• NCBI Accession #: NP_001545.2
• NCBI GenBank Nucleotide #: NM_001554.4
• UniProt Accession #: O00622; O14934; O43775; Q9BZL7
• Molecular Weight: 39,5 kDa
• NCBI Official Full Name: protein CYR61
• NCBI Official Synonym Full Names: cysteine-rich, angiogenic inducer, 61
• NCBI Official Symbol: CYR61
• NCBI Official Synonym Symbols: CCN1; GIG1; IGFBP10
• NCBI Protein Information: protein CYR61; IBP-10; IGFBP-10; CCN family member 1; IGF-binding protein 10; cysteine-rich, anigogenic inducer, 61; cysteine-rich heparin-binding protein 61; insulin-like growth factor-binding protein 10
• UniProt Protein Name: Protein CYR61
• Protein Family: Protein
• UniProt Gene Name: CYR61
• UniProt Synonym Gene Names: CCN1; GIG1; IGFBP10; IBP-10; IGF-binding protein 10; IGFBP-10
• UniProt Entry Name: CYR61_HUMAN

NCBI Description

The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]

Uniprot Description

Function: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. Ref.10

Subunit structure: Interaction with integrins is heparin- and cell-type-dependent and promotes cell adhesion. In skin fibroblasts it binds ITGA6/ITGB1, in endothelial cells, binds ITGAV/ITGB3 and in platelets, ITGA2B/ITGB3. Binds, in vitro, ITGAV/ITGB5.

Subcellular location: Secreted.

Sequence similarities: Belongs to the CCN family.Contains 1 CTCK (C-terminal cystine knot-like) domain.Contains 1 IGFBP N-terminal domain.Contains 1 TSP type-1 domain.Contains 1 VWFC domain.