Proheparin-Binding EGF-like Growth Factor Active Protein | HB-EGF active protein

Recombinant Human Proheparin-Binding EGF-like Growth Factor

Cat. Num. AAA144657

• Gene Names: HBEGF; DTR; DTS; DTSF; HEGFL
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Proheparin-Binding EGF-like Growth Factor; Recombinant Human Proheparin-Binding EGF-like Growth Factor; HB-EGF Human; Proheparin-Binding EGF-like Growth Factor Human Recombinant; Proheparin-binding EGF-like growth factor; HBEGF; DTR; DTS; HEGFL; HB-EGF; Heparin-binding EGF-like growth factor; Diphtheria toxin receptor; DT-R; DTSF; HB-EGF active protein

• Host: E Coli
• Purity/Purification: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: The protein was lyophilized from a concentrated (1mg/ml) solution containing 1xPBS pH-7.4.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL
• Sequence Length: 208

• Solubility: It is recommended to reconstitute the lyophilized Human HB-EGF in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1.0x106 units/mg.
• Preparation and Storage: Lyophilized Human HB-EGF Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution HB-EGF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for HB-EGF active protein

Description: HB-EGF Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids and having a molecular mass of 9.7kDa.The HB-EGF is purified by proprietary chromatographic techniques.

Introduction: HB-EGF is an EGF related growth factor which signals via the EGF receptor, and stimulates the proliferation of SMC (smooth muscle cells), fibroblasts, epithelial cells and keratinocytes. HB-EGF is expressed in various cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes and particular tumor cells. HB-EGF's ability to explicitly bind heparin and heparin sulfate proteoglycans is dissimilar from other EGF-like molecules, and might be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells.

Product Categories/Family for HB-EGF active protein

Cytokines and Growth Factors; Growth Factors; Epidermal Growth Factor

NCBI and Uniprot Product Information

• NCBI GI #: 4503413
• NCBI GeneID: 1839
• NCBI Accession #: NP_001936.1
• NCBI GenBank Nucleotide #: NM_001945.2
• UniProt Accession #: Q99075; B2R821
• Molecular Weight: 23,067 Da
• NCBI Official Full Name: proheparin-binding EGF-like growth factor
• NCBI Official Synonym Full Names: heparin-binding EGF-like growth factor
• NCBI Official Symbol: HBEGF
• NCBI Official Synonym Symbols: DTR; DTS; DTSF; HEGFL
• NCBI Protein Information: proheparin-binding EGF-like growth factor; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)
• UniProt Protein Name: Proheparin-binding EGF-like growth factor
• Protein Family: Proheparin-binding EGF-like growth factor
• UniProt Gene Name: HBEGF
• UniProt Synonym Gene Names: DTR; DTS; HEGFL; HB-EGF; HBEGF; DT-R
• UniProt Entry Name: HBEGF_HUMAN

Uniprot Description

HBEGF: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q23

Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: heparin binding; growth factor activity; epidermal growth factor receptor binding

Biological Process: positive regulation of keratinocyte migration; epidermal growth factor receptor signaling pathway; muscle development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; positive regulation of smooth muscle cell proliferation; negative regulation of elastin biosynthetic process; pathogenesis; signal transduction; positive regulation of cell growth; regulation of heart contraction; positive regulation of protein kinase B signaling cascade; positive regulation of cell proliferation; innate immune response; wound healing, spreading of epidermal cells; positive regulation of cell migration