Glutathione S-Transferase pi 1 Active Protein | GSTP1 active protein

Recombinant Mouse Glutathione S-Transferase pi 1

Cat. Num. AAA140661

• Gene Names: Gstp1; GstpiB
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Glutathione S-Transferase pi 1; Recombinant Mouse Glutathione S-Transferase pi 1; GSTP1 Mouse; Glutathione S-Transferase pi 1 Mouse Recombinant; Glutathione S-transferase P 1; Gst P1; GST YF-YF; GST class-pi; GST-piB; Preadipocyte growth factor; GSTP1 active protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: GSTP1 protein solution (1mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPPYTIV YFPVRGRCEA MRMLLADQGQ SWKEEVVTID TWMQGLLKPTCLYGQLPKFE DGDLTLYQSN AILRHLGRSL GLYGKNQREA AQMDMVNDGV EDLRGKYVTL IYTNYENGKNDYVKALPGHL KPFETLLSQN QGGKAFIVGD QISFADYNLLDLLLIHQVLA PGCLDNFPLL SAYVARLSAR PKIKAFLSSP EHVNRPINGNGKQ
• Sequence Length: 210

• Biological Activity: Specific activity is > 40 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for GSTP1 active protein

Introduction: GSTP1 is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are believed to function in xenobiotic metabolism and have a part in susceptibility to cancer, and other diseases. GSTP1 is a glutathione S-transferase belonging to the pi class. GST family enzymes play a significant role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based upon the biochemical, immunologic and structural properties of the soluble GSTs they are grouped into 4 main classes: alpha, mu, pi, and theta. The GSTP1 enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form a Sulfur-substituted glutathione. The reactions employing glutathione contribute the transformation of a broad range of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress.The GSTP1 inactivation through CpG hypermethylation is frequent in pituitary adenomas and may be a factor in aggressive pituitary tumor behavior. GSTP1 is may be a transcriptional target of the p53 tumor suppressor gene. Single-nucleotide polymorphism in GSTP1 is linked to modified protein binding, which influence GSTP1's contribution to carcinogen and drug metabolism, and possibly disease pathogenesis and/or drug response. GST-pi might have central roles in proliferation of androgen-independent human prostate cancer cells.

Description: GSTP1 Mouse Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 233 amino acids (1-210 a.a) and having a molecular mass of 26kDa. GSTP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Categories/Family for GSTP1 active protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 10092608
• NCBI GeneID: 14870
• NCBI Accession #: NP_038569.1
• NCBI GenBank Nucleotide #: NM_013541.1
• UniProt Accession #: P19157; Q8BNY4
• Molecular Weight: 23,609 Da
• NCBI Official Full Name: glutathione S-transferase P 1
• NCBI Official Synonym Full Names: glutathione S-transferase, pi 1
• NCBI Official Symbol: Gstp1
• NCBI Official Synonym Symbols: GstpiB
• NCBI Protein Information: glutathione S-transferase P 1
• UniProt Protein Name: Glutathione S-transferase P 1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: Gstp1
• UniProt Synonym Gene Names: Gstpib; Gst P1
• UniProt Entry Name: GSTP1_MOUSE

Uniprot Description

GSTP1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. Homodimer. Interacts with CDK5. Belongs to the GST superfamily. Pi family.

Protein type: EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Cellular Component: cytoplasm; cytosol; extracellular space; intracellular; mitochondrion; nucleus; plasma membrane; protein complex; vesicle

Molecular Function: drug binding; glutathione binding; glutathione peroxidase activity; glutathione transferase activity; JUN kinase binding; kinase regulator activity; protein kinase binding

Biological Process: glutathione metabolic process; negative regulation of apoptosis; negative regulation of biosynthetic process; negative regulation of fibroblast proliferation; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interleukin-1 beta production; negative regulation of JNK activity; negative regulation of nitric-oxide synthase biosynthetic process; negative regulation of stress-activated MAPK cascade; negative regulation of tumor necrosis factor production; positive regulation of superoxide release; regulation of stress-activated MAPK cascade; response to reactive oxygen species