FK506 Binding Protein 4 Active Protein | FKBP4 active protein

Recombinant Human FK506 Binding Protein 4

Cat. Num. AAA143842

• Gene Names: FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: FK506 Binding Protein 4; Recombinant Human FK506 Binding Protein 4; FKBP4 Human; FK506 Binding Protein 4 Human Recombinant; HBI; p52; Hsp56; FKBP52; FKBP59; PPIase; FKBP4; FK506-binding protein 4; Peptidyl-prolyl cis-trans isomerase; HSP-binding immunophilin; FKBP52 protein; 52 kDa FK506-binding protein; p59 protein; FKBP4 active protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The FKBP4 protein solution contains 20mM Tris-HCl, pH-8 and 10% glycerol.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MTAEEMKATE SGAQSAPLPM EGVDISPKQD EGVLKVIKRE GTGTEMPMIG DRVFVHYTGW LLDGTKFDSS LDRKDKFSFDLGKGEVIKAW DIAIATMKVG EVCHITCKPE YAYGSAGSPP KIPPNATLVF EVELFEFKGE DLTEEEDGGI IRRIQTRGEG YAKPNEGAIV EVALEGYYKDKLFDQRELRF EIGEGENLDL PYGLERAIQR MEKGEHSIVY LKPSYAFGSV GKEKFQIPPN AELKYELHLK SFEKAKESWE MNSEEKLEQS TIVKERGTVYFKEGKYKQAL LQYKKIVSWL EYESSFSNEE AQKAQALRLA SHLNLAMCHL KLQAFSAAIE SCNKALELDS NNEKGLFRRG EAHLAVNDFE LARADFQKVLQLYPNNKAAK TQLAVCQQRI RRQLAREKKL YANMFERLAE EENKAKAEAS SGDHPTDTEM KEEQKSNTAG SQSQVETEA
• Sequence Length: 459

• Biological Activity: Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH 8.0 using chymotrypsin.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for FKBP4 active protein

Description: FKBP4 produced in E Coli is a single,non-glycosylated polypeptide chain containing 479 amino acids (1-459 a.a.) and having a molecular mass of 53.9 kDa.FKBP4 is fused to 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

Introduction: FKBP4 is part of the immunophilin protein family, which takes part in immunoregulation and necessary cellular processes concerning protein folding and trafficking. FKBP4 is a cis-trans prolyl isomerase that connects to the immunosuppressants FK506 and rapamycin. FKBP4 has high structural and functional similarity to FKBP1A, though, FKBP4 does not have immunosuppressant activity when complexed with FK506. FKBP4 interacts with interferon regulatory factor-4 and is involved in immunoregulatory gene expression in B and T lymphocytes. FKBP4 is known to connect with phytanoyl-CoA alpha-hydroxylase. FKBP4 associates with HSP90 & HSP70 thus takes part in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. FKBP4 highly associates with adeno-associated virus type 2 vectors (AAV) resulting in a considerable increase in AAV-mediated transgene expression in human cell lines. FKBP4 is involved in the optimal use of AAV vectors in human gene therapy.

Product Categories/Family for FKBP4 active protein

Enzymes; FK506 Binding Protein

NCBI and Uniprot Product Information

• NCBI GI #: 4503729
• NCBI GeneID: 2288
• NCBI Accession #: NP_002005.1
• NCBI GenBank Nucleotide #: NM_002014.3
• UniProt Accession #: Q02790; Q9UCP1; Q9UCV7; D3DUQ1
• Molecular Weight: 51,805 Da
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase FKBP4
• NCBI Official Synonym Full Names: FK506 binding protein 4, 59kDa
• NCBI Official Symbol: FKBP4
• NCBI Official Synonym Symbols: HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase FKBP4; 51 kDa FK506-binding protein; FK506-binding protein 4 (59kD); HSP binding immunophilin; T-cell FK506-binding protein, 59kD; peptidylprolyl cis-trans isomerase; rotamase
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP4
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: FKBP4
• UniProt Synonym Gene Names: FKBP52; PPIase FKBP4; FKBP51; 52 kDa FKBP; FKBP-52; p59; FKBP-4; HBI
• UniProt Entry Name: FKBP4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

FKBP4: Immunophilin protein with PPIase and co-chaperone activities. Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.

Protein type: Chaperone; EC 5.2.1.8; Isomerase

Chromosomal Location of Human Ortholog: 12p13.33

Cellular Component: nucleoplasm; microtubule; endoplasmic reticulum membrane; mitochondrion; cell soma; perinuclear region of cytoplasm; cytoplasm; cytosol

Molecular Function: protein binding, bridging; protein binding; FK506 binding; peptidyl-prolyl cis-trans isomerase activity; GTP binding; heat shock protein binding; phosphoprotein binding; tau protein binding; copper-dependent protein binding; ATP binding; glucocorticoid receptor binding

Biological Process: prostate gland development; male sex differentiation; steroid hormone receptor complex assembly; protein complex localization; protein peptidyl-prolyl isomerization; protein folding; negative regulation of microtubule polymerization; androgen receptor signaling pathway; negative regulation of microtubule polymerization or depolymerization; copper ion transport; embryo implantation