Ribonuclease H Enzyme | Sta7437_3452 enzyme
Ribonuclease H (RNase H)
Purity
Purified
Synonyms
Ribonuclease H; Ribonuclease H (RNase H); Sta7437_3452 enzyme
Host
E Coli
Purity/Purification
Purified
Quality Control
Endonuclease/Nickase Activity: None detected after a 14-16 hour incubation of 5 units of enzyme with 300ng of supercoiled plasmid DNA, analyzed on an agarose gel.
Storage Buffer
100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100 and 50% glycerol.
Unit Definition
One unit of Ribonculease H will produce 1 nmol of acid-soluble ribonuleotides from radiolabeled poly(rA): poly (dT) in 20 minutes at 37 degree C.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for Sta7437_3452 enzyme
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Ribonuclease H
NCBI Official Symbol
Sta7437_3452
NCBI Protein Information
Ribonuclease H
Protein Family
Uniprot Description
Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.
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Product Notes
The Sta7437_3452 (Catalog #AAA654003) is an Enzyme produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Ribonuclease H, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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