b-Galactosidase Enzyme
b-Galactosidase (E. coli)
Purity
Molecular Biology GradePurified by chromatography.
Synonyms
b-Galactosidase; b-Galactosidase (E. coli); b-Galactosidase enzyme
Host
E Coli
Specificity
A review of substrate requirements is offered by Wallenfels and Malhotra (1960).
Purity/Purification
Molecular Biology Grade
Purified by chromatography.
Purified by chromatography.
Form/Format
Supplied as a suspension in 1.6M ammonium sulfate.
Activity
The reaction probably involves a galactosyl-enzyme intermediate (van de Groen 1973). See also Hill and Huber (1971) and Sinnott and Souchard (1973a and b). Hartl and Hall (1974) report a second beta-galactosidase without lactase activity in Vivo but with high ONPG (o-nitrophenyl, beta-D-galactopyranoside) activity. Monovalent cations have a stimulatory effect on the enzyme (Becker and Evans 1969). The alcohols, methanol, ethanol, i-propanol, and n-propanol, at 5% concentration, all increase the rate of o-nitrophenyl, beta]D-galactopyranoside cleavage (Shifrin and Hunn 1969). The enzyme is protected against heat-inactivation by 5-phosphorylribose 1-pyrophosphate in the presence of beta-mercaptoethanol (Moses and Sharp 1970).
Specific Activity
300 units per mg protein
Extinction Coefficient
20.9.
Note
This enzyme is not stable when diluted. All dilutions should be made as quickly as possible and used immediately.
Quality Control
SDS-PAGE
Unit Definition
1 unit hydrolyzes 1umole o-nitrophenyl-beta-D-galactopyranoside (ONPG) per min. at 25 degree C, pH 7.5 (Craven et al., 1965).
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for b-Galactosidase enzyme
Beta-galactosidases are widespread in microorganisms, animals and plants. That from the Escherichia coli strain K12 has been particularly studied at Anfinsen's laboratory in connection with genetic experiments on gene regulation of protein synthesis.
Beta-galactosidase is tetrameric, being composed of four identical subunits of 135kD, each with an active site which may be independently active (Melcher and Messe 1973). The enzyme is readily fragmented into small peptides (Marinkovic et al. 1975). The amino acid analysis indicates approximately 1170 residues per subunit (Fowler and Zabin 1970). See also Langley et al. (1975) and Naider et al. (1972). Kaneshiro et al. (1975) report on an active dimer.
Contaxis and Reithel (1974) report a small molecule with high 260nm absorbance which is bound to the enzyme in crude extracts as a stabilizer. On adding it to the pure enzyme, activity and heat stability are enhanced.
Bronskil and Wong (1971) suggest that the ribosomal enzyme may be an intermediate stage in the assembly of the quarternary structure.
Beta-galactosidase is tetrameric, being composed of four identical subunits of 135kD, each with an active site which may be independently active (Melcher and Messe 1973). The enzyme is readily fragmented into small peptides (Marinkovic et al. 1975). The amino acid analysis indicates approximately 1170 residues per subunit (Fowler and Zabin 1970). See also Langley et al. (1975) and Naider et al. (1972). Kaneshiro et al. (1975) report on an active dimer.
Contaxis and Reithel (1974) report a small molecule with high 260nm absorbance which is bound to the enzyme in crude extracts as a stabilizer. On adding it to the pure enzyme, activity and heat stability are enhanced.
Bronskil and Wong (1971) suggest that the ribosomal enzyme may be an intermediate stage in the assembly of the quarternary structure.
Product Categories/Family for b-Galactosidase enzyme
NCBI and Uniprot Product Information
Similar Products
Product Notes
The b-Galactosidase (Catalog #AAA653780) is an Enzyme produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "b-Galactosidase, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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