DNA Polymerase I DNA Manipulation
DNA Polymerase I (E. coli)
Applications
Polymerases for DNA Manipulation, RT-qPCR, RT-PCR and cDNA Synthesis, PCR
Synonyms
DNA Polymerase I; DNA Polymerase I (E. coli); DNA Polymerase I dna manipulation
Host
E Coli
Form/Format
25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25 degree C
Concentration
10,000 U/ml (varies by lot)
Applicable Applications for DNA Polymerase I dna manipulation
Polymerases for DNA Manipulation, RT-qPCR, RT-PCR and cDNA Synthesis, PCR
Source Note
An E Coli strain that carries an overexpressed copy of the polA gene.
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37 degree C.
Reaction Conditions
1X ABuffer B, Incubate at 37 degree C
Reaction Buffer or Mix
1X ABuffer B:
10 mM Tris-HC, 50 mM NaCl, 10 mM MgCl2, 1 mM DTT, pH7.9 at 25 degree C
10 mM Tris-HC, 50 mM NaCl, 10 mM MgCl2, 1 mM DTT, pH7.9 at 25 degree C
5' - 3' Exonuclease
Yes
3' - 5' Exonuclease
Yes
Strand Displacement
No
Error Rate
< 9x10^6 bases
Preparation and Storage
Store at -20 degree C.
Related Product Information for DNA Polymerase I dna manipulation
DNA Polymerase I (E coli) is a DNA-dependent DNA polymerase with inherent 3'--> 5' and 5'--> 3' exonuclease activities. The 5'--> 3' exonuclease activity removes nucleotides ahead of the growing DNA chain, allowing nick-translation. It is applicable to Nick translation of DNA to obtain probes with a high specific activity and second strand synthesis of cDNA.
Similar Products
Product Notes
The DNA Polymerase I (Catalog #AAA9141655) is a DNA Manipulation produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DNA Polymerase I can be used in a range of immunoassay formats including, but not limited to, Polymerases for DNA Manipulation, RT-qPCR, RT-PCR and cDNA Synthesis, PCR. Researchers should empirically determine the suitability of the DNA Polymerase I for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Polymerase I, DNA Manipulation" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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