Casein Kinase 2 beta Active Active Protein | CK2b active protein

Recombinant Human Casein Kinase 2 beta Active

Cat. Num. AAA142755

• Gene Names: CSNK2B; G5A; CK2B; CK2N; CSK2B
• Purity: Greater than 95% as determined by SDS-PAGE.
• Synonyms: Casein Kinase 2 beta Active; Recombinant Human Casein Kinase 2 beta Active; CSNK2B Human; Active; Casein Kinase 2 beta Human Recombinant; Casein kinase II subunit beta; CK II beta; Phosvitin; G5a; CK2B; CK2N; CSK2B; MGC138222; MGC138224; CK2b Active; CK2b active protein

• Host: E Coli
• Purity/Purification: Greater than 95% as determined by SDS-PAGE.
• Form/Format: CSNK2B lyophilized from 25mM Tris-HCl, 500mM NaCl, 1mM DTT, 1mM EDTA, protease inhibitor cocktail, pH 8.5.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence Length: 212

• Unit Defenition: 1U = 1 umol/min at 37 degree C) using the synthetic peptide RRRDDDSDDD.
• Solubility: Solubilize in distilled H2O or 50% glycerol.
• Biological Activity: Specific activity > 1U/mg.
• Preparation and Storage: Lyophilized CSNK2B although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CSNK2B should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Related Product Information for CK2b active protein

Description: Casein Kinase 2 beta Human Recombinant is a non-glycosylated polypeptide having a molecular mass of 24.9 kDa. Casein Kinase 2 is purified by proprietary chromatographic techniques.

Introduction: The Casein kinase 2 (EC2.7.11.1) is a serine/threonine-selective protein kinasethat is a tetramer of two alpha subunits and two beta subunits. The alpha subunits have the catalytic kinase domain. Casein kinase 2 has been implicated in cell cyclecontrol, DNA repair, regulation of the circadian rhythmand other cellular processes.Casein kinase 2 activity has been reported to be activated following Wnt signaling pathwayactivation. A Pertussis toxin-sensitive G proteinand Disheveled appear to be an intermediary between Wnt-mediated activation of the Frizzled receptor and activation of casein kinase 2.Mice that lack casein kinase 2 alpha prime have a defect in the morphology of developing sperm.

Product Categories/Family for CK2b active protein

Protein Kinases; Enzymes; Casein Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 538260597
• NCBI GeneID: 1460
• NCBI Accession #: NP_001269314.1
• NCBI GenBank Nucleotide #: NM_001282385.1
• UniProt Accession #: P67870; P07312; P13862; Q4VX47; B0UXA9
• Molecular Weight: 24,942 Da
• NCBI Official Full Name: casein kinase II subunit beta isoform 2
• NCBI Official Synonym Full Names: casein kinase 2, beta polypeptide
• NCBI Official Symbol: CSNK2B
• NCBI Official Synonym Symbols: G5A; CK2B; CK2N; CSK2B
• NCBI Protein Information: casein kinase II subunit beta; CK II beta; CSNK2B-LY6G5B-532; CSNK2B-LY6G5B-560; Casein kinase II beta subunit; alternative name: G5a, phosvitin; casein kinase II b subunit splicing isoform 318; casein kinase II b subunit splicing isoform 660; casein kinase II b subunit splicing isoform 806; chimera CSNK2B-LY6G5B splicing isoform 532; chimera CSNK2B-LY6G5B splicing isoform 560; protein G5a
• UniProt Protein Name: Casein kinase II subunit beta
• UniProt Gene Name: CSNK2B
• UniProt Synonym Gene Names: CK2N; G5A; CK II beta
• UniProt Entry Name: CSK2B_HUMAN

NCBI Description

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

Uniprot Description

CK2B: a regulatory subunit of the casein kinase 2 holoenzyme. Exists as a tetramer composed of two catalytic subunits, alpha and alpha-prime, and two regulatory beta subunits. The beta subunits undergo autophosphorylation. Plays a complex role in regulating the basal catalytic activity of the alpha subunit. Participates in Wnt signaling

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cytoplasm; plasma membrane; protein kinase CK2 complex; PcG protein complex; cytosol; nucleus

Molecular Function: protein domain specific binding; protein serine/threonine kinase activity; identical protein binding; protein binding; metal ion binding; protein kinase regulator activity; transcription factor binding; receptor binding

Biological Process: negative regulation of cell proliferation; axon guidance; positive regulation of activin receptor signaling pathway; Wnt receptor signaling pathway; cellular protein complex assembly; regulation of DNA binding; adiponectin-mediated signaling pathway; mitotic cell cycle; negative regulation of blood vessel endothelial cell migration; regulation of protein kinase activity; signal transduction; protein amino acid phosphorylation