RNase Inhibitor cDNA Synthesis
RNase Inhibitor, Mammalian
Applications
Cell-Free Protein Expression, PCR, RT-PCR, cDNA synthesis, In vitro transcription/translation, Enzymatic RNA labeling reaction, Other applications where the integrity of RNA is important
Synonyms
RNase Inhibitor; Mammalian; RNase Inhibitor cdna synthesis
Host
E Coli
Form/Format
20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol, pH7.6 at 25 degree C
Concentration
40,000 U/ml (varies by lot)
Applicable Applications for RNase Inhibitor cdna synthesis
Cell-Free Protein Expression, PCR, RT-PCR, cDNA synthesis, In vitro transcription/translation, Enzymatic RNA labeling reaction, Other applications where the integrity of RNA is important
Source Note
An E Coli strain that carries the Ribonuclease Inhibitor gene from Porcine.
Unit Definition
One unit is defined as the amount of RNase Inhibitor, Mammalian required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.
Preparation and Storage
Store at -20 degree C.
Related Product Information for RNase Inhibitor cdna synthesis
RNase Inhibitor, Mammalian specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).
The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 10^14.
The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 10^14.
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Product Notes
The RNase Inhibitor (Catalog #AAA9141651) is a cDNA Synthesis produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RNase Inhibitor can be used in a range of immunoassay formats including, but not limited to, Cell-Free Protein Expression, PCR, RT-PCR, cDNA synthesis, In vitro transcription/translation, Enzymatic RNA labeling reaction, Other applications where the integrity of RNA is important. Researchers should empirically determine the suitability of the RNase Inhibitor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNase Inhibitor, cDNA Synthesis" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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