CD40Ligand/TRAP Active Protein | CD40-L active protein

CD40Ligand / TRAP, murine recombinant

Cat. Num. AAA844635

• Gene Names: Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5
• Applications: SDS-Page
• Purity: >=98%
• Synonyms: CD40Ligand/TRAP; CD40Ligand / TRAP; murine recombinant; CD40-L; Tumor necrosis factor ligand superfamily member 5; TNF-related activation protein; TRAP; T cell antigen Gp39; CD154 antigen; sCD40; IGM; IMD3; HIGM1; T-BAM; TNFSF5; hCD40L.; CD40-L active protein

• Host: E Coli
• Purity/Purification: >=98%
• Form/Format: Sterile filtered and then lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 0.1 M Arginine.; Appearance: Lyophilized protein
• Sequence Length: 260

• Applicable Applications for CD40-L active protein: SDS-PAGE; HPLC
• Solubility/Reconstitution Instructions: Reconstitute in H?O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers or stored at 4 degree C for 1 week or -20 degree C for future use.
• Endotoxin Level: <0.1 ng/mug
• Biological Activity: Determined by its ability to induce TNF-alpha and MIP-1alpha production by murine splenocytes. The expected ED?? for this effect is <0.1 ug/ml.
• Results: <0.1 ug/ml.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for CD40-L active protein

Background: CD40 Ligand (CD40L/TRAP) is a type II transmembrane glycoprotein belonging to the TNF superfamily of ligands. Natually, CD40L is a membrane-bound protein, but proteolytically cleaved 15-18 kDa soluble forms of CD40L are also fully active. CD40L plays an important role in the proliferation and differentiation of B-cells, and is critical for immunoglobulin (Ig) class switching. Recombinant murine CD40 Ligand is a soluble 18 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CDE40L.

Product Categories/Family for CD40-L active protein

Cytokines; Growth Factors & Hormones; CDsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; CDs Proteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth

NCBI and Uniprot Product Information

• NCBI GI #: 15011846
• NCBI GeneID: 21947
• NCBI Accession #: NP_035746.2
• NCBI GenBank Nucleotide #: NM_011616.2
• UniProt Accession #: P27548
• Molecular Weight: 16.4 kDa
• NCBI Official Full Name: CD40 ligand
• NCBI Official Synonym Full Names: CD40 ligand
• NCBI Official Symbol: Cd40lg
• NCBI Official Synonym Symbols: IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5
• NCBI Protein Information: CD40 ligand
• UniProt Protein Name: CD40 ligand
• UniProt Gene Name: Cd40lg
• UniProt Synonym Gene Names: Cd40l; Tnfsf5; CD40-L; TRAP
• UniProt Entry Name: CD40L_MOUSE

Uniprot Description

CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.

Protein type: Cell adhesion; Membrane protein, integral

Cellular Component: extracellular space; cell surface; membrane; cell; plasma membrane; integral to membrane; extracellular region; external side of plasma membrane

Molecular Function: cytokine activity; CD40 receptor binding; tumor necrosis factor receptor binding

Biological Process: B cell proliferation; platelet activation; positive regulation of interleukin-12 production; isotype switching; positive regulation of immune response to tumor cell; signal transduction; positive regulation of interleukin-10 production; positive regulation of interferon-gamma production; immunoglobulin secretion; positive regulation of MHC class II biosynthetic process; B cell differentiation; positive regulation of B cell proliferation; immune response; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis