Calmodulin Active Protein | CALM1 active protein

Calmodulin, human recombinant

Cat. Num. AAA845406

• Gene Names: CALM2; caM; PHKD; CAMII; LQT15; PHKD2
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: Calmodulin; human recombinant; CaM; CALM Phosphodiesterase 3':5'-cyclic nucleotide activator; CALM2; PHKD; CAMII; PHKD2; phosphorylase kinase delta.; CALM1 active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: Lyophilized from a salt free solution.; Appearance: Lyophilized
• Sequence: MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
• Sequence Length: 197

• Applicable Applications for CALM1 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Reconstitute in water or an appropriate buffer (TBS, PBS, etc)
• Activity (Specifications/test method): The Ca2+ binding affinity of rh Calmodulin was evidenced by the electrophoretic mobility shift of Calmodulin in the presence of calcium and EDTA. Calmodulin has been shown to migrate differently in the presence and absence of Calcium. Ref.: Proc Natl Acad Sci U S A. 2004 Apr 6;101 (14):4787-92.
• Biological Activity: The Ca2+ binding affinity of rh Calmodulin was evidenced by the electrophoretic mobility shift of Calmodulin in the presence of calcium and EDTA. Calmodulin has been shown to migrate differently in the presence and absence of Calcium. Ref.: Proc Natl Acad Sci U S A. 2004 Apr 6;101 (14):4787-92.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for CALM1 active protein

Background: Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions. CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. Calmodulin is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. Calmodulin can also make use of the calcium stores in the endoplasmic reticulum, and the sarcoplasmic reticulum. CaM undergoes a conformational change upon binding to calcium, which enables it to bind to specific proteins for a specific response. CaM can bind up to four calcium ions, and can undergo post-translational modifications, such as phosphorylation, acetylation, methylation and proteolytic cleavage, each of which can potentially modulate its actions.

Product Categories/Family for CALM1 active protein

Proteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Other Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 779176942
• NCBI GeneID: 805
• NCBI Accession #: NP_001292553.1
• NCBI GenBank Nucleotide #: NM_001305624.1
• UniProt Accession #: P62158; P02593; P70667; P99014; Q13942; Q53S29; Q61379; Q61380; Q96HK3
• Molecular Weight: 16.8 kDa
• NCBI Official Full Name: calmodulin isoform 1
• NCBI Official Synonym Full Names: calmodulin 2 (phosphorylase kinase, delta)
• NCBI Official Symbol: CALM2
• NCBI Official Synonym Symbols: caM; PHKD; CAMII; LQT15; PHKD2
• NCBI Protein Information: calmodulin
• UniProt Protein Name: Calmodulin
• Protein Family: Calmodulin
• UniProt Gene Name: CALM1
• UniProt Synonym Gene Names: CALM; CAM; CAM1; CaM
• UniProt Entry Name: CALM_HUMAN

NCBI Description

This gene is a member of the calmodulin gene family. There are three distinct calmodulin genes dispersed throughout the genome that encode the identical protein, but differ at the nucleotide level. Calmodulin is a calcium binding protein that plays a role in signaling pathways, cell cycle progression and proliferation. Several infants with severe forms of long-QT syndrome (LQTS) who displayed life-threatening ventricular arrhythmias together with delayed neurodevelopment and epilepsy were found to have mutations in either this gene or another member of the calmodulin gene family (PMID:23388215). Mutations in this gene have also been identified in patients with less severe forms of LQTS (PMID:24917665), while mutations in another calmodulin gene family member have been associated with catecholaminergic polymorphic ventricular tachycardia (CPVT)(PMID:23040497), a rare disorder thought to be the cause of a significant fraction of sudden cardiac deaths in young individuals. Pseudogenes of this gene are found on chromosomes 10, 13, and 17. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

Uniprot Description

Calmodulin: a calcium-binding small regulatory protein that mediates the control of a large number of enzymes by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Has four functional calcium-binding domains. Phosphorylation and ubiquitination result in a decreased activity.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 14q32.11

Cellular Component: nucleoplasm; spindle pole; centrosome; sarcomere; growth cone; spindle microtubule; cytoplasm; extracellular region; plasma membrane; nucleus; cytosol; vesicle

Molecular Function: protein domain specific binding; nitric-oxide synthase regulator activity; titin binding; calcium ion binding; calcium-dependent protein binding; protein kinase binding; protein serine/threonine kinase activator activity; protein binding; N-terminal myristoylation domain binding; phospholipase binding; thioesterase binding; phosphoinositide 3-kinase binding; type 3 metabotropic glutamate receptor binding; nitric-oxide synthase binding; adenylate cyclase binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; phototransduction, visible light; inositol phosphate metabolic process; nerve growth factor receptor signaling pathway; pathogenesis; signal transduction; nitric oxide metabolic process; positive regulation of protein amino acid dephosphorylation; synaptic transmission; platelet degranulation; substantia nigra development; muscle contraction; positive regulation of cyclic nucleotide metabolic process; response to corticosterone stimulus; epidermal growth factor receptor signaling pathway; platelet activation; mitochondrion organization and biogenesis; fibroblast growth factor receptor signaling pathway; glycogen catabolic process; regulation of heart rate; organelle organization and biogenesis; positive regulation of nitric-oxide synthase activity; adenylate cyclase activation; response to amphetamine; glucose metabolic process; regulation of cytokinesis; positive regulation of protein amino acid autophosphorylation; rhodopsin mediated signaling; G-protein coupled receptor protein signaling pathway; regulation of rhodopsin mediated signaling; phospholipase C activation; carbohydrate metabolic process; innate immune response; positive regulation of cyclic-nucleotide phosphodiesterase activity; regulation of nitric-oxide synthase activity; detection of calcium ion; response to calcium ion; vascular endothelial growth factor receptor signaling pathway; blood coagulation

Disease: Long Qt Syndrome 14; Ventricular Tachycardia, Catecholaminergic Polymorphic, 4