Carbonic Anhydrase-1 Active Protein | CA1 active protein

Recombinant Human Carbonic Anhydrase-1, BioActive

Cat. Num. AAA141573

• Gene Names: CA1; CAB; CA-I; Car1
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Carbonic Anhydrase-1; Recombinant Human Carbonic Anhydrase-1; BioActive; CA1 Active; CA1 Human Active; Carbonic Anhydrase-1 Human Recombinant; CA1; CA-I; CAB; CA1 active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: Sterile filtered clear solution.; CA1 Human protein (1mg/ml) is formulated in 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol.
• Sequence: MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F

• Biological Activity: Specific activity is >300pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH8.0 at 37 degree C.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks.; Store, frozen at -20 degree C for longer periods of time.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Avoid multiple freeze-thaw cycles.

Related Product Information for CA1 active protein

CA, also known as carbonic anhydrase is an enzyme. Its main function revolves around the CO2 + H2O HCO3- + H+ (conversion of carbon dioxide to bicarbonate & protons). CA has a zinc ion in its active site. The main function of CA is to keep acid-base balance in the blood stream and various tissues. This enzyme also assists Carbonic Anhydrase I to move CO2 to and from tissues.
CA1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-261) and having a molecular mass of 31.0 kDa.
CA1 Humanis fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Categories/Family for CA1 active protein

ENZYMES

NCBI and Uniprot Product Information

• NCBI GI #: 192447430
• NCBI GeneID: 759
• NCBI Accession #: NP_001122301.1
• NCBI GenBank Nucleotide #: NM_001128829.2
• UniProt Accession #: P00915
• Molecular Weight: 28,870 Da
• NCBI Official Full Name: carbonic anhydrase 1
• NCBI Official Synonym Full Names: carbonic anhydrase I
• NCBI Official Symbol: CA1
• NCBI Official Synonym Symbols: CAB; CA-I; Car1
• NCBI Protein Information: carbonic anhydrase 1; carbonic anhydrase B; carbonic dehydratase; carbonate dehydratase I
• UniProt Protein Name: Carbonic anhydrase 1
• Protein Family: Cation-transporting ATPase
• UniProt Gene Name: CA1
• UniProt Synonym Gene Names: CAB; CA-I
• UniProt Entry Name: CAH1_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature. [provided by RefSeq, Sep 2009]

Uniprot Description

CA1: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Lyase; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: cytosol

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process