Insulin Like Growth Factor-1 Active Protein | bIGF1 active protein

Recombinant Rabbit Insulin Like Growth Factor-1

Cat. Num. AAA142303

• Gene Names: IGF1; IGF-IB
• Purity: Greater than 98.0% as determined by: (a) Gel-filtration chromatography under non denaturing conditions. (b) Analysis by SDS-PAGE.
• Synonyms: Insulin Like Growth Factor-1; Recombinant Rabbit Insulin Like Growth Factor-1; IGF1 Rabbit; Insulin-Like Growth Factor 1 Rabbit Recombinant; Somatomedin C; IGF-I; IGFI; IGF1; IGF-IA; Mechano growth factor; MGF; bIGF1 active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by: (a) Gel-filtration chromatography under non denaturing conditions. (b) Analysis by SDS-PAGE.
• Form/Format: The protein was lyophilized from a concentrated (0.5 mg/ml) solution with 0.02% NaHCO3.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MTAGPETLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKP
• Sequence Length: 143

• Solubility: It is recommended to reconstitute the lyophilized rbIGF-I in sterile 0.4% NaHCO3 adjusted, not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: IGF-I is biologically active when compared to human IGF-1. The ED50, calculated by the dose -dependent proliferation of human MCF/7 cells is 5 to 25ng/ml in the cell culture mixture dependent on culture conditions. Its activity consists of 30-40 % compared to human IGF-1.
• Preparation and Storage: Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IGF1 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for bIGF1 active protein

Description: Insulin-Like Growth Factor-I Rabbit Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 7639 Dalton.

Introduction: The IGFs comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone. Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin'. Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A, and somatomedin B. IGF-1 is a small protein secreted mostly but not exclusively by the liver and circulating in blood mostly as a complex with several IGF binding proteins. It has growth-regulating, insulin-like, and mitogenic functions and it is secreted in response to growth hormone stimulation.

Product Categories/Family for bIGF1 active protein

Cytokines and Growth Factors; Growth Factors; Insulin-Like Growth Factor

NCBI and Uniprot Product Information

• NCBI GI #: 126722801
• NCBI GeneID: 100008668
• NCBI Accession #: NP_001075495.1
• NCBI GenBank Nucleotide #: NM_001082026.1
• UniProt Accession #: Q95222; O18846
• Molecular Weight: 15,205 Da
• NCBI Official Full Name: insulin-like growth factor I
• NCBI Official Symbol: IGF1
• NCBI Official Synonym Symbols: IGF-IB
• NCBI Protein Information: insulin-like growth factor I; IGF-I; insulin-like growth factor IB; somatomedin
• UniProt Protein Name: Insulin-like growth factor I
• UniProt Gene Name: IGF1
• UniProt Synonym Gene Names: IGF-1; IGF-I
• UniProt Entry Name: IGF1_RABIT

Uniprot Description

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 ().