Twisted Gastrulation Protein Active Protein | TWSG1 active protein

Twisted Gastrulation Protein, Recombinant, Human (TSG)

Cat. Num. AAA650562

• Gene Names: TWSG1; TSG
• Purity: Highly Purified; 98% by SDS-PAGE and HPLC analyses.
• Synonyms: Twisted Gastrulation Protein; Recombinant; Human (TSG); TWSG1 active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 98% by SDS-PAGE and HPLC analyses.
• Form/Format: Supplied as a lyophilized powder in 10mM sodium acetate, pH6.0. Reconcentrate with dH2O, carrier protein to a concentration of 0.1-1mg/ml.

• Biological Activity: Determined by its ability to neutralize BMP-6 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is < 2.0 mg/ml of TSG.
• Country of Origin: USA
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, carrier protein, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for TWSG1 active protein

Twisted Gastrulation Protein (TSG) is a secreted BMP binding protein structurally related to the BMP antagonists Chordin and Noggin. TSG can inhibit BMP activity by binding directly to BMP proteins, and can act either as a BMP4 agonist or antagonist (depending on the specific biochemical environment) by binding to the BMP4/Chordin complex. Recombinant human TSG is a 199 amino acid 22kD protein containing the BMP/TGF binding portion of the full length TSG protein.

Product Categories/Family for TWSG1 active protein

Growth Factors; Cytokines; Growth Factors-Other

NCBI and Uniprot Product Information

• NCBI GI #: 10190664
• NCBI GeneID: 57045
• NCBI Accession #: NP_065699.1
• NCBI GenBank Nucleotide #: NM_020648.5
• UniProt Accession #: Q9GZX9; Q8NBI7; Q96K46; B2RE08; D3DUH9
• Molecular Weight: 22kD
• NCBI Official Full Name: twisted gastrulation protein homolog 1
• NCBI Official Synonym Full Names: twisted gastrulation homolog 1 (Drosophila)
• NCBI Official Symbol: TWSG1
• NCBI Official Synonym Symbols: TSG
• NCBI Protein Information: twisted gastrulation protein homolog 1; OTTHUMP00000162352
• UniProt Protein Name: Twisted gastrulation protein homolog 1
• Protein Family: Twisted gastrulation protein
• UniProt Gene Name: TWSG1
• UniProt Synonym Gene Names: TSG
• UniProt Entry Name: TWSG1_HUMAN

Uniprot Description

Function: May be involved in dorsoventral axis formation. Seems to antagonize BMP signaling by forming ternary complexes with CHRD and BMPs, thereby preventing BMPs from binding to their receptors. In addition to the anti-BMP function, also has pro-BMP activity, partly mediated by cleavage and degradation of CHRD, which releases BMPs from ternary complexes. May be an important modulator of BMP-regulated cartilage development and chondrocyte differentiation. May play a role in thymocyte development

Subunit structure: Interacts with CHRD and BMP4. This interaction enhances CHRD/BMP4 complex formation. Interacts with BMP7

Subcellular location: Secreted

Developmental stage: Expressed in brain throughout development. Ref.3

Domain: The N-terminal domain is sufficient to interact with BMP4

Sequence similarities: Belongs to the twisted gastrulation protein family.