SULT1B1 active protein

SULT1B1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120865

• Gene Names: SULT1B1; ST1B1; ST1B2; SULT1B2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: SULT1B1; SULT1B1 Protein; Human; Recombinant (His Tag); Human SULT1B1/ST1B2 Protein (His Tag); ST1B1 Protein; ST1B2 Protein; SULT1B2 Protein; sulfotransferase family; cytosolic; 1B; member 1; SULT1B1 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 0.1 M NaCl, 10% glycerol, 1mM DTT, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Leu2-Ile296

• Species: Human
• Activity: Measured by its ability to transfer sulfate from PAPS to 1-Napthol. The specific activity is >40pmoles/min/ug.
• Predicted N Terminal: Met 1
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human SULT1B1 (NP_055280.2)(Leu 2-Ile 296) was expressed, with a N-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SULT1B1 active protein

Background: Sulfotransferase family cytosolic 1B member 1, also known as Sulfotransferase 1B1, Sulfotransferase 1B2, Thyroid hormone sulfotransferase, SULT1B1 and ST1B2, is a cytoplasm protein that belongs to the sulfotransferase 1 family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SULT1B1 is highly expressed in the liver, peripheral blood leukocytes, colon (mucosal lining), small intestine (jejunum) and spleen. A lesser expression of SULT1B1 was observed in the lung, placenta and thymus. SULT1B1 catalyzes the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. SULT1B1 sulfates dopamine, small phenols such as 1-naphthol and p-nitrophenol and thyroid hormones, including 3,3'-diiodothyronine, triidothyronine, reverse triiodothyronine and thyroxine.

References

Fujita K. et al., 1997, J. Biochem. 122:1052-61. Kester,MH.et al., 2003,Am J Physiol Endocrinol Metab. 285 (3):E592-8. Meinl W, et al., 2001, Biochem Biophys Res Commun. 288 (4): 855-62. Dombrovski L. et al., 2006, Proteins 64: 1091-4.

NCBI and Uniprot Product Information

• NCBI GI #: 29550928
• NCBI GeneID: 27284
• NCBI Accession #: NP_055280.2
• NCBI GenBank Nucleotide #: NM_014465.3
• UniProt Accession #: O43704; O15497; Q96FI1; Q9UK34
• Molecular Weight: 34,899 Da
• NCBI Official Full Name: sulfotransferase family cytosolic 1B member 1
• NCBI Official Synonym Full Names: sulfotransferase family, cytosolic, 1B, member 1
• NCBI Official Symbol: SULT1B1
• NCBI Official Synonym Symbols: ST1B1; ST1B2; SULT1B2
• NCBI Protein Information: sulfotransferase family cytosolic 1B member 1; sulfotransferase 1B1; sulfotransferase 1B2; thyroid hormone sulfotransferase
• UniProt Protein Name: Sulfotransferase family cytosolic 1B member 1
• Protein Family: Sulfotransferase family cytosolic
• UniProt Gene Name: SULT1B1
• UniProt Synonym Gene Names: ST1B2; SULT1B2; ST1B1; Sulfotransferase 1B1; ST1B2
• UniProt Entry Name: ST1B1_HUMAN

NCBI Description

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. However, the total genomic length of this gene is greater than that of other SULT1 genes. [provided by RefSeq, Jul 2008]

Uniprot Description

SULT1B1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates dopamine, small phenols such as 1-naphthol and p-nitrophenol and thyroid hormones, including 3,3'-diiodothyronine, triidothyronine, reverse triiodothyronine and thyroxine. Belongs to the sulfotransferase 1 family.

Protein type: Transferase; EC 2.8.2.-

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: cytosol

Molecular Function: sulfotransferase activity; aryl sulfotransferase activity

Biological Process: steroid metabolic process; biogenic amine metabolic process; flavonoid metabolic process; epithelial cell differentiation; xenobiotic metabolic process; sulfation; 3'-phosphoadenosine 5'-phosphosulfate metabolic process; thyroid hormone metabolic process; phenol metabolic process