Sulfotransferase 1E1 Active Protein
Sulfotransferase 1E1, Recombinant, Human (SULT1E1, estrogen-preferring, member 1, EST, EST-1, Estrogen sulfotransferase, MGC34459, STE)
Applications
Western Blot
Purity
>95% by SDS-PAGE under reducing conditions and visualized by silver stain.
Synonyms
Sulfotransferase 1E1; Recombinant; Human (SULT1E1; estrogen-preferring; member 1; EST; EST-1; Estrogen sulfotransferase; MGC34459; STE); Sulfotransferase 1E1 active protein
Host
E.coli
Purity/Purification
>95% by SDS-PAGE under reducing conditions and visualized by silver stain.
Form/Format
Supplied as a liquid in 25mM Tris, pH 7.5, 250mM sodium chloride, 2.5mM DTT.
Concentration
0.88mg/ml (varies by lot)
Applicable Applications for Sulfotransferase 1E1 active protein
Western Blot (WB) - Other applications not tested.
Application Notes
Optimal dilutions to be determined by the researcher.
Host Note
Recombinant enzyme corresponding to Asn2-Ile294 with an N-terminal 6 His tag of human Sulfotransferase 1E1 expressed in E. coli.
Endotoxin
<1EU/ug (LAL).
N-terminal Sequence Analysis
His
Activity
Measured by its ability to transfer sulfate from PAPS to 1Napthol.
Specific Activity
>40pmol/min/ug
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for Sulfotransferase 1E1 active protein
Cytosolic Sulfotransferases catalyze the sulfation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm. SULT1E1 is widely known as an estrogen sulfotransferase and may control estrogen levels by converting free estradiol to its inactive sulfate conjugate (1). Known substrates of this enzyme also include dehydroepiandrosterone, pregnenolone, ethinyl estradiol , equalenin, diethylstilbesterol , 4nitrophenol, 1napthol and resveratrol (2,3). SULT1E1 activity is found in reproductive organs (4), intestine and liver (3,5).
Product Categories/Family for Sulfotransferase 1E1 active protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Sulfotransferase 1E1 (Catalog #AAA636493) is an Active Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Sulfotransferase 1E1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB) - Other applications not tested. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Sulfotransferase 1E1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sulfotransferase 1E1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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