Stem Cell Factor Active Protein

Stem Cell Factor, Recombinant, Mouse (Stem Cell Factor, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)

Cat. Num. AAA650726

• Purity: Highly Purified; Highly purified (98% by SDS-PAGE and HPLC analyses).
• Synonyms: Stem Cell Factor; Recombinant; Mouse (Stem Cell Factor; CD117 c-kit; Steel Factor; Mast Cell Growth Factor; MGF); Stem Cell Factor active protein

• Host: E Coli
• Purity/Purification: Highly Purified; Highly purified (98% by SDS-PAGE and HPLC analyses).
• Form/Format: Supplied as a lyophilized powder from 10mM sodium phosphate, pH 6.5. Reconstitute with sterile dH2O to a concentration of 0.1-1.0mg/ml with carrier protein (0.1% HSA or BSA).

• Biological Activity: The ED50, determined by the dose-dependent stimulation of the proliferation of the human TF-1 cells is; 10ng/ml corresponding to a specific activity of 1x10e5u/mg. For most in vitro applications, SCF exerts its biological activity in the range of 1-100ng/ml.
• Country of Origin: USA
• Endotoxin Level: 0.1ng/ug (1EU/ug)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer or ddH2O and carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Stem Cell Factor active protein

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Murine SCF is an 18.3kD protein containing 164 amino acid residues (secreted soluble form).

Product Categories/Family for Stem Cell Factor active protein

Growth Factors; Cytokines; Growth Factors-Cell Factors

NCBI and Uniprot Product Information

• NCBI GI #: 1246100
• Molecular Weight: 18.3kD
• NCBI Official Full Name: stem cell factor

NCBI Description

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: extracellular space; cytoskeleton; cytoplasm; plasma membrane; extracellular region; integral to membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; stem cell factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; embryonic hemopoiesis; germ cell programmed cell death; male gonad development; signal transduction; cell proliferation; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of melanocyte differentiation; negative regulation of mast cell apoptosis; innate immune response; positive regulation of Ras protein signal transduction; positive regulation of myeloid leukocyte differentiation; cell adhesion; neural crest cell migration; positive regulation of DNA replication

Disease: Hyperpigmentation, Familial Progressive, 2; Skin/hair/eye Pigmentation, Variation In, 7