SCF active protein

SCF, 26-189 aa, Mouse, E Coli (Bioactivity Validated)

Cat. Num. AAA206268

• Gene Names: Kitl; Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; blz; Kitlg; contrasted
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: SCF; 26-189 aa; Mouse; E Coli (Bioactivity Validated); Stem cell factor; Kitl; Clo; Con; contrasted; Gb; Kitlg; Mgf; SF; Sl; SLF; Steel; SCF active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffer saline (pH 7.4) containing 10% glycerol.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
• Sequence Length: 273

• Applicable Applications for SCF active protein: SDS-PAGE
• Antigen Species: Mouse
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 for this effect is less or equal to 20 ng/ml.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SCF active protein

Stem Cell Factor (SCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. SCF binds to and activates the SCF receptor (SCFR), a receptor tyrosine kinase. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell-cell adhesion and acts synergistically with other cytokine. Recombinant mouse SCF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Categories/Family for SCF active protein

Metabolism; Signal Transduction

References

Zhang Z., et al. (2000). Proc. Natl. Acad. Sci. U.S.A. 97, 7732.; Okada S, et al. (1992). Nippon Rinsho, 50, 1872.

NCBI and Uniprot Product Information

• NCBI GI #: 7305267
• NCBI GeneID: 17311
• NCBI Accession #: NP_038626
• NCBI GenBank Nucleotide #: NM_013598.3
• UniProt Accession #: P20826; P97332; Q3TNJ7; Q62524; Q64222; Q921N5
• Molecular Weight: 18 kDa (165 aa), confirmed by MALDI-TOF
• NCBI Official Full Name: kit ligand isoform 1
• NCBI Official Synonym Full Names: kit ligand
• NCBI Official Symbol: Kitl
• NCBI Official Synonym Symbols: Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; blz; Kitlg; contrasted
• NCBI Protein Information: kit ligand
• UniProt Protein Name: Kit ligand
• Protein Family: SCF-associated factor
• UniProt Gene Name: Kitlg
• UniProt Synonym Gene Names: Kitl; Mgf; Sl; Slf; MGF; SCF; sKITLG

Uniprot Description

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.