RANTES Active Protein | CCL5 active protein

RANTES, Recombinant, Mouse (Regulated on Activation, Normal T-cell Expressed and Secreted)

Cat. Num. AAA650837

• Gene Names: CCL5; SISd; SCYA5; RANTES; TCP228; D17S136E; MGC17164
• Purity: Highly Purified; 95% as determined by RP-HPLC and SDS-PAGE. Endotoxin: 0.1ng/ug (1EU/ug).
• Synonyms: RANTES; Recombinant; Mouse (Regulated on Activation; Normal T-cell Expressed and Secreted); CCL5 active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% as determined by RP-HPLC and SDS-PAGE. Endotoxin: 0.1ng/ug (1EU/ug).
• Form/Format: Supplied as a lyophilized powder without additives. Reconstitute with sterile ddH2O to a concentration 0.1mg/ml. Working dilutions can be made in aqueous buffer.

• Biological Activity: Recombinant Rantes is fully biologically active when compared to standard. The activity is determined by the ability to chemoattract total human lymphocytes and murine T-cells at a concentration between 1-10ng/ml.
• Preparation and Storage: Store desiccated below -20 degree C. Upon reconstitution, the liquid can be stored at degree 4C between 2-7 days and for future use, below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze-thaw cycles.

Related Product Information for CCL5 active protein

Recombinant Mouse Rantes produced in E. coli is a single, non-glycosylated polypeptide chain containing 68 amino acids and having a molecular mass of 7876 Dalton. Recombinant murine Rantes is purified by proprietary chromatographic techniques.

Product Categories/Family for CCL5 active protein

Growth Factors; Cytokines; Growth Factors-RANTES

NCBI and Uniprot Product Information

• NCBI GI #: 2905632
• NCBI GeneID: 6352
• UniProt Accession #: P13501; O43646; Q0QVW8; Q4ZGJ1; Q9NYA2; Q9UBG2; Q9UC99; Q9UBL2
• Molecular Weight: 7.88kD
• NCBI Official Full Name: RANTES
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 5
• NCBI Official Symbol: CCL5
• NCBI Official Synonym Symbols: SISd; SCYA5; RANTES; TCP228; D17S136E; MGC17164
• NCBI Protein Information: C-C motif chemokine 5; eoCP; SIS-delta; OTTHUMP00000163880; OTTHUMP00000197106; beta-chemokine RANTES; small-inducible cytokine A5; T-cell specific protein p288; t cell-specific protein P228; T-cell-specific protein RANTES; eosinophil chemotactic cytokine; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; regulated upon activation, normally T-expressed, and presumably secreted
• UniProt Protein Name: C-C motif chemokine 5
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL5
• UniProt Synonym Gene Names: D17S136E; SCYA5
• UniProt Entry Name: CCL5_HUMAN

NCBI Description

This gene is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. [provided by RefSeq]

Uniprot Description

Function: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. Ref.3 Ref.8 Ref.10 Ref.11 Ref.12

Subcellular location: Secreted.

Tissue specificity: T-cell and macrophage specific.

Induction: By mitogens.

Post-translational modification: N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in Ref.8. They are assigned by similarity.

Polymorphism: The variant Phe-24 is an antagonist of the chemokine receptors CCR1 and CCR3.

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

Mass spectrometry: Molecular mass is 7515±1 Da from positions 27 - 91. Determined by SELDI. Ref.12Molecular mass is 7862.8±1.1 Da from positions 24 - 91. Determined by ESI. Ref.8Molecular mass is 8355±10 Da from positions 24 - 91. Determined by ESI. O-glycosylated. Ref.8