PTP1B active protein

PTP1B (human), (recombinant)

Cat. Num. AAA566621

• Gene Names: PTPN1; PTP1B
• Purity: >=95% (SDS-PAGE); Purified by multi-step chromatography
• Synonyms: PTP1B; PTP1B (human); (recombinant); PTP1B active protein

• Host: E Coli
• Purity/Purification: >=95% (SDS-PAGE); Purified by multi-step chromatography
• Form/Format: Liquid. In 50mM HEPES, pH7.2, containing 1mM EDTA, 5mM DTT, and 0.05% NP-40

• Source Note: Human PTP1B (protein tyrosine phosphatase 1B) (aa 1-322)
• Specific Activity: >=20uMol/min/mg at 30 degree C using 0.15mM of phosphopeptide substrate based on EGFR residues 988-998 in 50mM HEPES, pH7.2, 1mM EDTA, 1mM DTT, 0.05% NP-40, and 0.2ug/ul BSA
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: For long term storage, store at -80 degree C. Avoid freeze/thaw cycles; Shipping: Shipped on Dry Ice

Related Product Information for PTP1B active protein

PTP1B is the ubiquitous, prototypical nontransmembrane PTP originally identified in human placenta.

Product Categories/Family for PTP1B active protein

Proteostasis; Posttranslational Modifications

NCBI and Uniprot Product Information

• NCBI GI #: 4506289
• NCBI GeneID: 5770
• NCBI Accession #: NP_002818.1
• NCBI GenBank Nucleotide #: NM_002827.2
• UniProt Accession #: P18031; Q5TGD8; Q9BQV9; Q9NQQ4
• Molecular Weight: 49,967 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 1
• NCBI Official Symbol: PTPN1
• NCBI Official Synonym Symbols: PTP1B
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 1; protein tyrosine phosphatase 1B; protein-tyrosine phosphatase 1B; protein tyrosine phosphatase, placental
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 1
• UniProt Gene Name: PTPN1
• UniProt Synonym Gene Names: PTP1B; PTP-1B
• UniProt Entry Name: PTN1_HUMAN

NCBI Description

The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. [provided by RefSeq, Jul 2008]

Uniprot Description

PTP1B: a non-receptor phospho-tyrosine protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation and inactivation of PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. Negatively regulates insulin signaling by dephosphorylating the phosphotyrosine residues of insulin receptor. Also reported to dephosphorylate integrin, epidermal growth factor receptor, JAK2 and TYK2, regulating cell growth control and the cellular response to interferon. May regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes.

Protein type: Protein phosphatase, tyrosine (non-receptor); Phosphatase; EC 3.1.3.48; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q13.1-q13.2

Cellular Component: endoplasmic reticulum; early endosome; plasma membrane; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; enzyme binding; ephrin receptor binding; zinc ion binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; protein kinase binding; insulin receptor binding

Biological Process: platelet activation; unfolded protein response, activation of signaling protein activity; negative regulation of vascular endothelial growth factor receptor signaling pathway; unfolded protein response; cytokine and chemokine mediated signaling pathway; regulation of endocytosis; actin cytoskeleton reorganization; insulin receptor signaling pathway; regulation of signal transduction; negative regulation of insulin receptor signaling pathway; blood coagulation

Disease: Diabetes Mellitus, Noninsulin-dependent