pituitary growth hormone 20K Active Protein | GH1 active protein
Human pituitary growth hormone 20K
Gene Names
GH1; GH; GH-N
Purity
The purity of recombinant pituitary human growth hormone20K is greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 9.
(c) Analysis by reducing an
Synonyms
pituitary growth hormone 20K; Human pituitary growth hormone 20K; Human pituitary growth hormone 20 K; GH1 active protein
Host
E Coli
Purity/Purification
The purity of recombinant pituitary human growth hormone
20K is greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 9.
(c) Analysis by reducing an
20K is greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 9.
(c) Analysis by reducing an
Form/Format
Sterile filtered white lyophilized (freeze-dried) powder.
Sequence
The sequence of the first five N-terminal amino acids of recombinant pituitary human growth hormone 20K was determined and was found to be Ala-Phe-Pro-Thr-Ile.
Sequence Length
217
Solubility
It is recommended to reconstitute the lyophilized recombinant pituitary human growth hormone 20K in 0.4% NaHCO3 adjusted to pH 10, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation of recombinant pituitary human growth hormone 20Kwas carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.735 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of GH-20K as a Reference Standard.
1. UV spectroscopy at 280 nm using the absorbency value of 0.735 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of GH-20K as a Reference Standard.
Dimers and Aggregates
Less than 1% as determined by gel filtration chromatography of recombinant pituitary human growth hormone 20K under non denaturative conditions.
Endotoxin
Less than 0.1 ng/ug (IEU/ug) of recombinant pituitary human growth hormone 20K.
Formulation
The recombinant pituitary human growth hormone 20K was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 previously adjusted pH 10.
Biological Activity
Recombinant pituitary human growth hormone 20K is fully biologically active when compared to World Health Organization (WHO) reference standard as evidenced by several in vitro bioassays. Recombinant pituitary human growth hormone 20K is also capable of forming a 1:2 complex with the recombinant human growth hormone receptor extracellular domain (ECD).
Preparation and Storage
Lyophilized recombinant pituitary human growth hormone
20K although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution and filter sterilization recombinant pituitary human growth hormone 20Kcan be stored at 4 degree C, pH 10 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
20K although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution and filter sterilization recombinant pituitary human growth hormone 20Kcan be stored at 4 degree C, pH 10 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Related Product Information for GH1 active protein
Recombinant pituitary human growth hormone - 20K produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids and having a molecular mass of 20322 Dalton. Recombinant pituitary human growth hormone
20K is purified by proprietary chromatographic techniques (see Solomon et al. Growth Horm IGF Res. 2006;16:297-307).
20K is purified by proprietary chromatographic techniques (see Solomon et al. Growth Horm IGF Res. 2006;16:297-307).
Product Categories/Family for GH1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
24,843 Da
NCBI Official Full Name
pituitary growth hormone
NCBI Official Symbol
GH1
NCBI Official Synonym Symbols
GH; GH-N
NCBI Protein Information
somatotropin; Pituitary growth hormone
UniProt Protein Name
Somatotropin
Protein Family
UniProt Gene Name
GH1
UniProt Synonym Gene Names
GH; GH-N
UniProt Entry Name
SOMA_PANTR
Uniprot Description
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues ().
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Product Notes
The GH1 gh1 (Catalog #AAA400049) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids of recombinan t pituitary human growth hormone 20K was determined and was found to be Ala-Phe-Pr o-Thr-Ile. It is sometimes possible for the material contained within the vial of "pituitary growth hormone 20K, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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