Peroxiredoxin 3 Active Protein | PRDX3 active protein

Peroxiredoxin 3, 63-256aa, Human, E Coli (Bioactivity Validated)

Cat. Num. AAA203322

• Gene Names: PRDX3; AOP1; MER5; AOP-1; SP-22; HBC189; PRO1748; prx-III
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Peroxiredoxin 3; 63-256aa; Human; E Coli (Bioactivity Validated); Thioredoxin-dependent peroxide reductase mitochondrial; PRDX3; PRX III; Antioxidant protein 1 (AOP-1); Thioredoxin-dependent peroxide reductase; mitochondrial Antioxidant Protein 1; AOP1; MER5; PRX3; SP22; Peroxiredoxin-3; PRDX3 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MPAVTQHAPY FKGTAVVNGE FKDLSLDDFK GKYLVLFFYP LDFTFVCPTE IVAFSDKANE FHDVNCEVVA VSVDSHFSHL AWINTPRKNG GLGHMNIALL SDLTKQISRD YGVLLEGSGL ALRGLFIIDP NGVIKHLSVN DLPVGRSVEE TLRLVKAFQY VETHGEVCPA NWTPDSPTIK PSPAASKEYF QKVNQ
• Sequence Length: 256

• Applicable Applications for PRDX3 active protein: SDS-PAGE
• Antigen Species: Human
• Biological Activity: Specific activity is >2,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PRDX3 active protein

Peroxiredoxin 3, also known as PRDX3, is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Peroxiredoxin 3 is specifically localized in mitochondria and believed to play important roles in the regulation of cellular redox status by serving as a primary line of defense against H2O2 produced during respiration. Recombinant human Peroxiredoxin 3 protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PRDX3 active protein

Redox Proteins

References

Cox AG., et al. (2009). Biochemistry. 48 (27):6495-501; Rhee SG., et al. (2005). Free Radic Biol Med. 38 (12):1543-52;

NCBI and Uniprot Product Information

• NCBI GI #: 5802974
• NCBI GeneID: 10935
• NCBI Accession #: NP_006784
• NCBI GenBank Nucleotide #: NM_006793.4
• UniProt Accession #: P30048; P35690; Q0D2H1; Q13776; Q5T5V2; Q96HK4; B2R7Z0; D3DRC9; E9PH29
• Molecular Weight: 21.5 kDa (195aa), confirmed by MALDI-TOF
• NCBI Official Full Name: thioredoxin-dependent peroxide reductase, mitochondrial isoform a
• NCBI Official Synonym Full Names: peroxiredoxin 3
• NCBI Official Symbol: PRDX3
• NCBI Official Synonym Symbols: AOP1; MER5; AOP-1; SP-22; HBC189; PRO1748; prx-III
• NCBI Protein Information: thioredoxin-dependent peroxide reductase, mitochondrial
• UniProt Protein Name: Thioredoxin-dependent peroxide reductase, mitochondrial
• Protein Family: Thioredoxin-dependent peroxide reductase
• UniProt Gene Name: PRDX3
• UniProt Synonym Gene Names: AOP1; AOP-1; Prx-III

NCBI Description

This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22. [provided by RefSeq, Oct 2014]

Uniprot Description

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (PubMed:7733872, PubMed:17707404). Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol (PubMed:12492477).