NMNAT3 active protein

NMNAT3 (human), (recombinant)

Cat. Num. AAA566070

• Gene Names: NMNAT3; PNAT3; FKSG76; PNAT-3
• Purity: >=95% (SDS-PAGE)
• Synonyms: NMNAT3; NMNAT3 (human); (recombinant); NMNAT3 active protein

• Host: E Coli
• Purity/Purification: >=95% (SDS-PAGE)
• Form/Format: Liquid. In 50mM TRIS-HCl, pH8.0, containing 300mM sodium chloride, 1mM DTT and 10% glycerol

• Source Note: Full length human NMNAT3 is fused at the N-terminus to a His-tag
• Specific Activity: >=2U/mg. One U=1uMol/min at 30 degree C. One unit will catalyze the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: For long term storage, store at -80 degree C. Avoid freeze/thaw cycles; Shipping: Shipped on Blue Ice

Related Product Information for NMNAT3 active protein

Nicotinamide mononucleotide adenyltransferase (NMNAT) catalyzes the formation of nicotinamide adenine dinucleotide (NAD) from nicotinamide mononucleotide (NMN) and ATP. Three NMNAT isoforms (NMNAT 1-3) have been identified with different kinetic parameters and cellular localizations. In humans, NMNAT1 is widely expressed in all tissues, while NMNAT2 and NMNAT3 show a tissue-specific expression and whose mRNA levels are generally lower compared to NMNAT1. NMNAT3 is more strongly expressed in tissues where NMNAT2 is almost absent. While NMNAT1 protein is localized in the nucleus, and NMNAT2 protein is localized in the cytosol, golgi apparatus and endoplasmic reticulum specifically, the NMNAT3 protein is localized in the mitochondria and less is known about it. Human NMNAT3 amino acid sequence is 50% identical to NMNAT1 and 34% identical to NMNAT2. Even though the 3D structure of the NMNAT3 monomer is almost identical to NMNAT1, NMNAT3 forms a tetramer while NMNAT1 forms a hexamer. It has been suggested that NMNAT2 forms a homodimer. The enzyme could be a potential target for therapeutical applications, because its activity is rather low in tumor cells.

Product Categories/Family for NMNAT3 active protein

Cellular Analysis; DNA Regulation & Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 318068039
• NCBI GeneID: 349565
• NCBI Accession #: NP_001186976.1
• NCBI GenBank Nucleotide #: NM_001200047.1
• UniProt Accession #: Q96T66; Q8N4G1; B3KVR6; D3DNF2; D3DNF3
• Molecular Weight: ~27.0 kDa (monomer). Human full-length NMNAT3 (aa 1-252) is fused at the N-terminus to a His-tag.
• NCBI Official Full Name: nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3 isoform 2
• NCBI Official Synonym Full Names: nicotinamide nucleotide adenylyltransferase 3
• NCBI Official Symbol: NMNAT3
• NCBI Official Synonym Symbols: PNAT3; FKSG76; PNAT-3
• NCBI Protein Information: nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3
• UniProt Protein Name: Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3
• Protein Family: Nicotinamide/nicotinic acid mononucleotide adenylyltransferase
• UniProt Gene Name: NMNAT3
• UniProt Synonym Gene Names: NMN/NaMN adenylyltransferase 3; NMN adenylyltransferase 3; NaMN adenylyltransferase 3; PNAT-3
• UniProt Entry Name: NMNA3_HUMAN

NCBI Description

This gene encodes a member of the nicotinamide/nicotinic acid mononucleotide adenylyltransferase family. These enzymes use ATP to catalyze the synthesis of nicotinamide adenine dinucleotide or nicotinic acid adenine dinucleotide from nicotinamide mononucleotide or nicotinic acid mononucleotide, respectively. The encoded protein is localized to mitochondria and may also play a neuroprotective role as a molecular chaperone. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

NMNAT3: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD (+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Protects against axonal degeneration following injury. Belongs to the eukaryotic NMN adenylyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Apoptosis; Transferase; EC 2.7.7.1; EC 2.7.7.18; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: mitochondrion; cytosol

Molecular Function: nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity; ATP binding

Biological Process: NAD biosynthetic process; vitamin metabolic process; NAD metabolic process; response to wounding; water-soluble vitamin metabolic process