Rabbit anti-Human, Mouse SHB Polyclonal Antibody | anti-SHB antibody

SHB Antibody

Cat. Num. AAA9605949

• Gene Names: SHB; bA3J10.2
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: SHB; Polyclonal Antibody; SHB Antibody; bA3J10.2; OTTHUMP00000021397; RP11 3J10.8; RP113J10.8; SH2 domain containing adapter protein B; SH2 domain-containing adapter protein B; SHB (Src homology 2 domain containing) adaptor protein B; SHB adaptor protein (a Src homology 2 protein); SHB adaptor protein; Shb; SHB_HUMAN; Src homology 2 domain containing adaptor protein B; Src homology 2 protein; anti-SHB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SHB antibody detects endogenous levels of total SHB
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 509

• Applicable Applications for anti-SHB antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide
• Subcellular Location: Cytoplasm. Cell Membrane. Associates with membrane lipid rafts upon TCR stimulation.
• Tissue Specificity: Widely expressed.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HuvEc cells, using SHB antibody.The lane on the left is treated with the antigen-specific peptide.)

Immunofluorescene (IF)

(MBS9605949 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Related Product Information for anti-SHB antibody

Function: Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells.
Subunit Structure: Interacts with PTPN11 (By similarity). Interacts with phosphorylated 'Tyr-720' of the ligand-activated receptor PDGFRA via its SH2 domain. Interacts with the ligand-activated receptors PDGFRB, FGFR1, KDR/VEGFR2, IL2RB and IL2RG. Interacts with EPS8 and V-SRC. Interacts with GRB2 and GRAP. Interacts with CD3Z. Interacts with tyrosine-phosphorylated LAT upon T-cell antigen receptor activation. Interacts with PLCG1. Interacts with ZAP70, LCP2/SLP-76, VAV1 and GRAP2. Interacts with JAK1 and JAK3. Interacts with PTK2/FAK1. Interacts with CRK/CrKII. Interacts with IRS2.
Post-translational Modifications: Phosphorylated upon PDGFRA, PDGFRB, TCR, IL2 receptor, FGFR1 or VEGFR2 activation.
Similarity: The SH2 domain preferentially binds phosphopeptides with the consensus sequence Y-[TVI]-X-L and mediates interaction with PDGFRA, PDGFRB, FGRFR1, IL2RB, IL2RG, CD3Z and CRK/CrKII.

NCBI and Uniprot Product Information

• NCBI GI #: 106879210
• NCBI GeneID: 6461
• NCBI Accession #: NP_003019.2
• NCBI GenBank Nucleotide #: NM_003028.2
• UniProt Accession #: Q15464; Q504U5; Q5VUM8; B9EGM0; D3DRQ5
• Molecular Weight: Observed: 55 kD; Predicted: 56 kDa
• NCBI Official Full Name: SH2 domain-containing adapter protein B
• NCBI Official Synonym Full Names: SH2 domain containing adaptor protein B
• NCBI Official Symbol: SHB
• NCBI Official Synonym Symbols: bA3J10.2
• NCBI Protein Information: SH2 domain-containing adapter protein B
• UniProt Protein Name: SH2 domain-containing adapter protein B
• Protein Family: SH2 domain-containing adapter protein
• UniProt Gene Name: SHB

Uniprot Description

Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells.