Myosin Light Chain 2, Non-muscle Active Protein | MYH14 active protein

Myosin Light Chain 2, Non-muscle, Recombinant, Human (MLC2, Myosin Regulatory Light Chain, MRLC, RLC, LC20)

Cat. Num. AAA636682

• Gene Names: MYH14; DFNA4; MHC16; MYH17; PNMHH; DFNA4A; myosin; FP17425; NMHC II-C; NMHC-II-C
• Purity: Highly Purified; ~90% by SDS-PAGE
• Synonyms: Myosin Light Chain 2; Non-muscle; Recombinant; Human (MLC2; Myosin Regulatory Light Chain; MRLC; RLC; LC20); MYH14 active protein

• Host: E Coli
• Purity/Purification: Highly Purified; ~90% by SDS-PAGE
• Form/Format: Supplied as a liquid in 20mM HEPES-KOH, pH 7.0, 50mM sodium chloride, 1mM EDTA and 1mM DTT.
• Sequence: MSSKKAKTKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID KEDLHDMLAS LGKNPTDAYL DAMMNEAPGP INFTMFLTMF GEKLNGTDPE DVIRNAFACF DEEATGTIQE DYLRELLTTM GDRFTDEEVD ELYREAPIDK KGNFNYIEFT RILKHGAKDK DD

• Application Notes: Suitable for use in ELISA, Western Blot and Inhibition Assays.
• Activity: Assayed for phosphorylation by MLCK.
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and freeze at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Related Product Information for MYH14 active protein

Non muscle Myosins are required for cytokinesis at the end of cell division, when a contractile ring near the plasmalemma divides the cytoplasm of the daughter cells. They are involved in cytoplasmic streaming movements in tissues, and especially in activation of motile cells such as fibroblasts and macrophages. Activation of non-muscle myosin-II is achieved by phosphorylation of Ser33 in the motif KKRPQRATSN by a dedicated, Ca+2 Calmodulin regulated light chain kinase (MLCK).

Product Categories/Family for MYH14 active protein

Molecular Biology; MB-Myosin

NCBI and Uniprot Product Information

• NCBI GI #: 116284394
• NCBI GeneID: 79784
• NCBI Accession #: NP_079005.3
• NCBI GenBank Nucleotide #: NM_024729.3
• UniProt Accession #: Q7Z406; Q5CZ75; Q6XYE4; Q76B62; Q8WV23; Q96I22; Q9BT27; Q9BW35; Q9H882; B0I1S2; C3TTN4
• Molecular Weight: 227,871 Da
• NCBI Official Full Name: myosin-14 isoform 2
• NCBI Official Synonym Full Names: myosin, heavy chain 14, non-muscle
• NCBI Official Symbol: MYH14
• NCBI Official Synonym Symbols: DFNA4; MHC16; MYH17; PNMHH; DFNA4A; myosin; FP17425; NMHC II-C; NMHC-II-C
• NCBI Protein Information: myosin-14; MYH14 variant protein; myosin, heavy polypeptide 14; non-muscle myosin heavy chain IIc; nonmuscle myosin heavy chain II-C; myosin heavy chain, non-muscle IIc
• UniProt Protein Name: Myosin-14
• Protein Family: Myosin
• UniProt Gene Name: MYH14
• UniProt Synonym Gene Names: KIAA2034; NMHC II-C
• UniProt Entry Name: MYH14_HUMAN

NCBI Description

This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-14 (MYO14). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene result in one form of autosomal dominant hearing impairment. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

MYH14: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). High levels of expression are found in small intestine, colon and skeletal muscle. Expression is low in organs composed mainly of smooth muscle, such as aorta, uterus and urinary bladder. No detectable expression is found in thymus, spleen, placenta and lymphocytes. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: growth cone; membrane; axon; myosin II complex; cytoplasm; stress fiber; actomyosin

Molecular Function: calmodulin binding; actin filament binding; microfilament motor activity; actin-dependent ATPase activity; ATP binding

Biological Process: skeletal muscle contraction; axon guidance; regulation of cell shape; skeletal muscle development; sensory perception of sound; metabolic process; actin filament-based movement; actomyosin structure organization and biogenesis; ephrin receptor signaling pathway; generation of action potential

Disease: Peripheral Neuropathy, Myopathy, Hoarseness, And Hearing Loss; Deafness, Autosomal Dominant 4a