MCAF, Rat Active Protein

MCAF, Recombinant, Rat (MCP-1, Macrophage/Monocyte Chemotactic and Activating Factor)

Cat. Num. AAA650273

• Purity: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Synonyms: MCAF; Rat; Recombinant; Rat (MCP-1; Macrophage/Monocyte Chemotactic and Activating Factor); Rat active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Form/Format: Supplied as a sterile filtered liquid in 20mM citrate, pH 3.5. No stabilizers added.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Val.

• Biological Activity: MCP-1 is fully biologically active when compared to standard. The specific activity as determined by the ability of Rat MCP-1 to chemoattract human peripheral blood at 10-100ng/ml.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, add 0.1% BSA or HSA, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

Related Product Information for MCAF, Rat active protein

Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). It is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor kappa B (NFkappaB). CCL2 recruits immune cells, such as monocytes, to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23aa and a mature peptide of 76aa. It is a monomeric polypeptide, with a molecular weightof approximately 13kD. As with many other CC chemokines, CCL2 is located on chromosome 17 in humans. The cell surface receptors that bind CCL2 are CCR2 and CCR5

Recombinant Rat MCP-1 is a non-glycosylated, Polypeptide chain containing 125aa and having a molecular mass of 14.09kD.

Product Categories/Family for MCAF, Rat active protein

Growth Factors; Cytokines; Growth Factors-MCAF

NCBI and Uniprot Product Information

• NCBI GI #: 29611342
• Molecular Weight: 14.09kD
• NCBI Official Full Name: MBD1-containing chromatin associated factor

NCBI Description

ATF7IP is a multifunctional nuclear protein that associates with heterochromatin. It can act as a transcriptional coactivator or corepressor depending upon its binding partners (summary by Liu et al., 2009 [PubMed 19106100]).[supplied by OMIM, Nov 2010]

Uniprot Description

ATF7IP: Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3). The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3). Facilitates telomerase TERT and TERC gene expression by SP1 in cancer cells. Belongs to the MCAF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12p13.1

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; ATPase activity; transcription corepressor activity

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; viral reproduction; positive regulation of transcription, DNA-dependent; DNA methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of transcriptional preinitiation complex assembly