Macrophage Inflammatory Protein 3 beta Active Protein | CCL19 active protein

Macrophage Inflammatory Protein 3 beta, Recombinant, Human (MIP-3b, Exodus-3, ELC)

Cat. Num. AAA650989

• Gene Names: CCL19; ELC; CKb11; MIP3B; MIP-3b; SCYA19; MGC34433
• Purity: Molecular Biology Grade; 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug)
• Synonyms: Macrophage Inflammatory Protein 3 beta; Recombinant; Human (MIP-3b; Exodus-3; ELC); CCL19 active protein

• Host: E Coli
• Purity/Purification: Molecular Biology Grade; 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug)
• Form/Format: Supplied as a lyophilized powder from a sterile filtered solution, lyophilized without additives. Reconstitute with sterile dH2O to 0.1-1.0mg/ml. This solution can then be diluted into other buffers.
• Sequence: GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS

• Biological Activity: Determined by its ability to chemoattract human T cells using a concentration of 10-50ng/ml.
• Reconstitution: Reconstitute with sterile dH2O to 0.1-1.0mg/ml. This solution can then be diluted into other buffers and stored at 4 degree C for 1 week or stored at -20 degree C for future use.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CCL19 active protein

MIP-3 beta is a CC chemokine that is expressed in the thymus, lymph nodes and in activated bone marrow stromal cells and signals through the CCR7 receptor. MIP-3 beta is a chemoattractant for T and B lymphocytes and myeloid progenitor cells. Human MIP-3 beta is active on murine cells. Recombinant human MIP-3 beta is an 8.8kD protein containing 77 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Product Categories/Family for CCL19 active protein

Growth Factors; Cytokines; Growth Factors-MIP

NCBI and Uniprot Product Information

• NCBI GI #: 1791003
• NCBI GeneID: 6363
• UniProt Accession #: Q6IBD6; Q99731
• Molecular Weight: 8.8kD
• NCBI Official Full Name: macrophage inflammatory protein 3 beta
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 19
• NCBI Official Symbol: CCL19
• NCBI Official Synonym Symbols: ELC; CKb11; MIP3B; MIP-3b; SCYA19; MGC34433
• NCBI Protein Information: C-C motif chemokine 19; exodus-3; CK beta-11; MIP-3-beta; OTTHUMP00000021295; OTTHUMP00000021296; EBI1 ligand chemokine; EBI1-ligand chemokine; CC chemokine ligand 19; beta chemokine exodus-3; beta-chemokine exodus-3; small-inducible cytokine A19; macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; epstein-Barr virus-induced molecule 1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19
• UniProt Protein Name: CCL19 protein
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL19
• UniProt Entry Name: Q6IBD6_HUMAN

NCBI Description

This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq]

Uniprot Description

CCL19: May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: extracellular space; extracellular region

Molecular Function: CCR chemokine receptor binding; chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding; CCR7 chemokine receptor binding

Biological Process: formation of immunological synapse; positive regulation of interleukin-12 production; positive regulation of dendritic cell antigen processing and presentation; cell maturation; positive regulation of JNK cascade; positive regulation of NF-kappaB import into nucleus; positive regulation of endocytosis; positive regulation of interleukin-1 beta secretion; positive regulation of receptor-mediated endocytosis; activation of JNK activity; dendritic cell chemotaxis; cell communication; positive regulation of T cell proliferation; establishment of T cell polarity; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of chemotaxis; response to virus; positive regulation of phosphoinositide 3-kinase activity; positive regulation of tumor necrosis factor production; cellular calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; myeloid dendritic cell chemotaxis; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; T cell costimulation; immune response; positive regulation of T-helper 1 cell differentiation