Specific Activity
(The specific activity of KRAS was determined to be 3 nmol/min/mg as per activity assay protocol.)
KRAS Active Protein
KRAS (Mature form), Active
Purity
>90%
Synonyms
KRAS; KRAS (Mature form); Active; KRAS active protein
Host
E Coli
Purity/Purification
>90%
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Concentration
0.1 ug/ul (varies by lot)
Specific Activity
3 nmol/min/mg
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at –70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Specific Activity
(The specific activity of KRAS was determined to be 3 nmol/min/mg as per activity assay protocol.)
Specific Activity
(The specific activity of KRAS was determined to be 3 nmol/min/mg as per activity assay protocol.)
SDS-Page
(The purity of KRAS was determined to be >90% by densitometry. Approx. MW 23kDa.)
SDS-Page
(The purity of KRAS was determined to be >90% by densitometry. Approx. MW 23kDa.)
Related Product Information for KRAS active protein
Recombinant human KRAS (2-186) was expressed in E.coli cells using an N-terminal His tag. The gene accession number is NM_033360.
The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). RAS and several other cellular proteins with similar biochemical properties are collectively known as G-proteins and they play key roles in a wide variety of cellular activities, including cell growth, differentiation, secretion, and protein trafficking (1). There are three forms of RAS gene in cells termed H-RAS, N-RAS, and K-RAS. RAS proteins play a direct causal role in human cancer and in other diseases. Mutant H-RAS, N-RAS, and K-RAS occur in varying frequencies in different tumor types (2). Other members of the RAS superfamily may also contribute to cancer.
The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). RAS and several other cellular proteins with similar biochemical properties are collectively known as G-proteins and they play key roles in a wide variety of cellular activities, including cell growth, differentiation, secretion, and protein trafficking (1). There are three forms of RAS gene in cells termed H-RAS, N-RAS, and K-RAS. RAS proteins play a direct causal role in human cancer and in other diseases. Mutant H-RAS, N-RAS, and K-RAS occur in varying frequencies in different tumor types (2). Other members of the RAS superfamily may also contribute to cancer.
Product Categories/Family for KRAS active protein
References
1.Shih, T. Y., et al: Structure and function of p21 ras proteins. Gene Amplif Anal. 1986;4:53-72.
2.Rodriguez-Viciana, P.: Cancer targets in the Ras pathway. Cold Spring Harb Symp Quant Biol. 2005;70:461-7.
2.Rodriguez-Viciana, P.: Cancer targets in the Ras pathway. Cold Spring Harb Symp Quant Biol. 2005;70:461-7.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The KRAS (Catalog #AAA515783) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KRAS, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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