Interferon tau, Ovine Active Protein | IFNT2 active protein

Interferon tau, Recombinant, Ovine (IFNt)

Cat. Num. AAA650027

• Gene Names: IFNT2; IFNT; TP-1; IFNT1; IFN-tau-c2
• Purity: Highly Purified; 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
• Synonyms: Interferon tau; Ovine; Recombinant; Ovine (IFNt); IFNT2 active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
• Form/Format: Supplied as a lyophilized powder in 0.5X PBS, pH 7.4.

• Biological Activity: Ovine IFN-Tau is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 5.7 x 107 IU/mg.
• Dimers and Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
• Endotoxin Level: 0.1ng/ug (IEU/ug) of Recombinant Ovine Interferon-Tau.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280 nm using the absorbency value of 1.027 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).; 2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Ovine IFN-Tau as a Reference Standard.
• Solubility: It is recommended to reconstitute the lyophilized interferon-Tau in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: -20 degree C; Stability: Lyophilized Recombinant Ovine Tau Interferon although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IFN-Tau should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Related Product Information for IFNT2 active protein

Interferon-tau is also known as TP-1 (trophoblast protein-1) is a new class of type I IFN that is secreted by the trophoblast and is the signal for maternal recognition of pregnancy in sheep. Interferon-tau has potent immunosuppressive and antiviral activities similar to other type I IFN but is less cytotoxic than IFN-alpha and IFN-beta. The current investigation concerns the effect of recombinant ovine IFN- tau (Recombinant Ovine IFN- tau) on the modulation of MHC class I and II expression on cloned mouse cerebrovascular endothelial (CVE) cells. IFN-tau induced tyrosine phosphorylation of Stat1 and upregulated the expression of MHC class I on CVE. One proposed action by which type I IFN reduces the relapse rate in MS is via interference with IFN-g-induced MHC class II expression. Interferon tau was shown to downregulate IFN-gamma-induced MHC class II expression on CVE and, hence, may be of potential therapeutic value in downregulating inflammation in the central nervous system (CNS). Interferon- tau did not upregulate the expression of MHC class II on CVE. Interferon- tau also inhibited the replication of Theiler's virus in CVE.

Recombinant Ovine Interferon-Tau produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of ~20kD. Recombinant Ovine IFN-Tau is purified by proprietary chromatographic techniques.

Product Categories/Family for IFNT2 active protein

Growth Factors; Cytokines; Growth Factors-Interferon

NCBI and Uniprot Product Information

• NCBI GI #: 269973909
• NCBI GeneID: 317698
• NCBI Accession #: NP_001015511.3
• NCBI GenBank Nucleotide #: NM_001015511.3
• UniProt Accession #: P15696; P15694; Q28126; Q28127; Q28128; Q28191; Q95NE2; P56830
• Molecular Weight: ~20kD
• NCBI Official Full Name: interferon tau-2
• NCBI Official Symbol: IFNT2
• NCBI Official Synonym Symbols: IFNT; TP-1; IFNT1; IFN-tau-c2
• NCBI Protein Information: interferon tau-2; IFN-tau1; IFN-tau2; IFN-tau-1; IFN-tau-2; trophoblastin; antiluteolysin; interferon tau-1; interferon-tau1e; interferon-tau3e; interferon alpha II; trophoblast protein 1; trophoblast protein-1; interferon, trophoblast; trophoblast type I interferon; trophoblast antiluteolytic protein
• UniProt Protein Name: Interferon tau-1
• Protein Family: Interferon
• UniProt Gene Name: IFNT1
• UniProt Synonym Gene Names: IFN-tau-1; TP-1
• UniProt Entry Name: IFNT1_BOVIN

Uniprot Description

Function: Paracrine hormone primarily responsible for maternal recognition of pregnancy. Interacts with endometrial receptors, probably type I interferon receptors, and blocks estrogen receptor expression, preventing the estrogen-induced increase in oxytocin receptor expression in the endometrium. This results in the suppression of the pulsatile endometrial release of the luteolytic hormone prostaglandin F2-alpha, hindering the regression of the corpus luteum (luteolysis) and therefore a return to ovarian cyclicity. This, and a possible direct effect of IFN-tau on prostaglandin synthesis, leads in turn to continued ovarian progesterone secretion, which stimulates the secretion by the endometrium of the nutrients required for the growth of the conceptus. In summary, displays particularly high antiviral and antiproliferative potency concurrently with particular weak cytotoxicity, high antiluteolytic activity and immunomodulatory properties. In contrast with other IFNs, IFN-tau is not virally inducible.

Subcellular location: Secreted. Note: Secreted into the uterine lumen.

Tissue specificity: Constitutively and exclusively expressed in the mononuclear cells of the extraembryonic trophectoderm.

Developmental stage: Major secretory product synthesized by the bovine conceptus between days 15 and 25 of pregnancy.

Polymorphism: There seems to be four variants of IFN-tau 1: A, B (shown here), C and D.

Miscellaneous: IFN-tau genes are intronless. They evolved from IFN-omega genes in the ruminantia suborder and have continued to duplicate independently in different lineages of the ruminantia. They code for proteins very similar in sequence but with different biological potency and pattern of expression.

Sequence similarities: Belongs to the alpha/beta interferon family. IFN-alphaII subfamily.