Insulin Active Protein | INS active protein

Insulin protein

Cat. Num. AAA538034

• Gene Names: INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
• Applications: User optimized
• Purity: > 98% (by SDS-PAGE and RP-HPLC)
• Synonyms: Insulin; Insulin protein; Insulin recombinant protein; INS active protein

• Host: E. Coli.
• Purity/Purification: > 98% (by SDS-PAGE and RP-HPLC)
• Form/Format: Sterile filtered lyophilized (freeze-dried) powder, from a concentration of 1mg/ml solution with no additives. Reconstitute in sterile 0.005N HCI not more than 1.0mg/ml.
• Concentration: 25mg per vial: lyophilized (varies by lot)
• Sequence Length: 110

• Applicable Applications for INS active protein: User optimized
• Species: Human
• Grade: Highly Pure
• Bioactivity: 28 IU/mg
• Residues: Insulin Human Recombinant protein containing 51 amino acids.
• Protein Type: Recombinant
• Method of Purification: Insulin protein was purified by a proprietary chromatographic technique.
• Biological Significance: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
• Preparation and Storage: Upon receipt, store at -20°C for best results. Stable at room temperature for 3 weeks.; Should be stored at -20°C after reconstitution. For long term storage it is recommended to add a carrier protein such as 0.1% HSA or BSA. Avoid freeze-thaw cycles.

Related Product Information for INS active protein

Purified recombinant Human Insulin protein

Product Categories/Family for INS active protein

Hormones & Steroids

NCBI and Uniprot Product Information

• NCBI GI #: 386828
• NCBI GeneID: 3630
• UniProt Accession #: P01308; Q5EEX2
• Molecular Weight: 5807 Da
• NCBI Official Full Name: insulin
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
• NCBI Protein Information: insulin
• UniProt Protein Name: Insulin
• Protein Family: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_HUMAN

NCBI Description

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Uniprot Description

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family.

Protein type: Hormone; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; endoplasmic reticulum lumen; Golgi lumen; extracellular region

Molecular Function: identical protein binding; insulin-like growth factor receptor binding; protein binding; protease binding; hormone activity; insulin receptor binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of vasodilation; glucose homeostasis; activation of NF-kappaB transcription factor; positive regulation of glucose import; negative regulation of protein oligomerization; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; activation of protein kinase B; positive regulation of nitric-oxide synthase activity; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of cell differentiation; negative regulation of protein catabolic process; regulation of amino acid metabolic process; negative regulation of glycogen catabolic process; wound healing; positive regulation of glycogen biosynthetic process; positive regulation of cellular protein metabolic process; glucose transport; regulation of protein secretion; negative regulation of vasodilation; positive regulation of MAPKKK cascade; negative regulation of lipid catabolic process; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; negative regulation of fatty acid metabolic process; alpha-beta T cell activation; endocrine pancreas development; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; energy reserve metabolic process; insulin receptor signaling pathway; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cell migration; positive regulation of cytokine secretion

Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10