IL16 active protein

Recombinant Human IL16

Cat. Num. AAA4159851

• Gene Names: IL16; LCF; NIL16; PRIL16; prIL-16
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IL16; Recombinant Human IL16; Lymphocyte chemoattractant factor; IL16 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS
• Sequence Length: 1331

• Bioactivity: The ED50 is 7.5-37.5 ng/mL as determined by its ability to chemoattract human CD4+ T lymphocytes (at a concentration range of 1.0-100.0 ng/mL).
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: Reconstitute in sterile PBS at 5ug/mL
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Related Product Information for IL16 active protein

Interleukin 16 (IL16) is a pleiotrophic cytokine derived from CD8+ T-cells that stimulates chemotaxis in CD4+ lymphocytes, monocytes, and eosinophils. It primes CD4+ T-cells for IL2 and IL15 responsiveness, induces T-lymphocyte Interleukin 2 (IL2) receptor expression, and inhibits HIV replication. The signalling process of IL16 is mediated by CD4, and undergoes proteolysis to yield two functional proteins. IL16 isoform 1 has the possible function of acting as a scaffolding protein to anchor ion channels in the membrane, while isoform 3 is involved in cell cycle progression in T-cells and transcriptional regulation of SKP2. IL16 mRNA expression levels have been found in various tissues such as spleen, bone marrow, thymus, and lymph nodes.

NCBI and Uniprot Product Information

• NCBI GI #: 223460532
• NCBI GeneID: 3603
• UniProt Accession #: Q14005
• Molecular Weight: 13.5 kDa
• NCBI Official Full Name: IL16 protein
• NCBI Official Synonym Full Names: interleukin 16
• NCBI Official Symbol: IL16
• NCBI Official Synonym Symbols: LCF; NIL16; PRIL16; prIL-16
• NCBI Protein Information: pro-interleukin-16
• UniProt Protein Name: Pro-interleukin-16
• Protein Family: Pro-interleukin
• UniProt Gene Name: IL16
• UniProt Synonym Gene Names: IL-16; LCF
• UniProt Entry Name: IL16_HUMAN

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Cytokine; DNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: extracellular space; cytoplasm; plasma membrane; nucleus

Molecular Function: cytokine activity

Biological Process: viral reproduction; regulation of transcription, DNA-dependent; transcription, DNA-dependent; leukocyte chemotaxis; immune response; induction of positive chemotaxis