IL-1beta Active Protein | IL1B2 active protein
Recombinant Porcine Interleukin-1 beta (rPoIL-1beta)
Gene Names
IL1B2; IL1B; IL-1beta
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Synonyms
IL-1beta; Recombinant Porcine Interleukin-1 beta (rPoIL-1beta); IL1B2 active protein
Host
E.coli
Purity/Purification
> 95 % by SDS-PAGE and HPLC analyses.
Form/Format
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 3% trehalose.
Sequence
ANVQSMECKL QDKDHKSLVL AGPHMLKALH LLTGDLKREV VFCMSFVQGD DSNNKIPVTL GIKGKNLYLS CVMKDNTPTL QLEDIDPKRY PKRDMEKRFV FYKTEIKNRV EFESALYPNW YISTSQAEQK PVFLGNSKGR QDITDFTMEV LSP
Sequence Length
267
Biological Activity
Fully biologically active when compared to standard. The EDso as determined by a cell proliferation assay using murine DI0S cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 x 10^5 IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Endotoxin
Less than 1 EU/ug of rPoIL-1beta as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < = -20 degree C. Further dilutions should be made in appropriate buffered solutions.
Molecular Weight
Approximately 17.6kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Preparation and Storage
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°c. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°c.
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
Related Product Information for IL1B2 active protein
Porcine Interleukin-l beta
Interleukin-l beta (IL-beta) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-lalpha and IL-1Beta binds to the same receptor and has similar but not identical biological properties. Recombinant porcine interleukin-l beta is a 17 kDa protein containing 153 amino acid residues and it shares 63%-70% a.a. sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL-1beta.
Interleukin-l beta (IL-beta) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-lalpha and IL-1Beta binds to the same receptor and has similar but not identical biological properties. Recombinant porcine interleukin-l beta is a 17 kDa protein containing 153 amino acid residues and it shares 63%-70% a.a. sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL-1beta.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
interleukin 1, beta
NCBI Official Symbol
IL1B2
NCBI Official Synonym Symbols
IL1B; IL-1beta
NCBI Protein Information
interleukin 1, beta
Research Articles on IL1B2
Similar Products
Product Notes
The IL1B2 (Catalog #AAA546469) is an Active Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: ANVQSMECKL QDKDHKSLVL AGPHMLKALH LLTGDLKREV VFCMSFVQGD DSNNKIPVTL GIKGKNLYLS CVMKDNTPTL QLEDIDPKRY PKRDMEKRFV FYKTEIKNRV EFESALYPNW YISTSQAEQK PVFLGNSKGR QDITDFTMEV LSP. It is sometimes possible for the material contained within the vial of "IL-1beta, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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