IL-1 beta / IL1B active protein

Recombinant Human IL-1 beta / IL1B Protein (pro form, His tag)

Cat. Num. AAA2546492

• Gene Names: IL1B; IL-1; IL1F2; IL1-BETA
• Purity: >95 % as determined by reducing SDS-PAGE.
• Synonyms: IL-1 beta / IL1B; Recombinant Human IL-1 beta / IL1B Protein (pro form; His tag); IL-1; IL-1 beta; IL-1B; IL1-BETA; IL1F2; IL-1 beta / IL1B active protein

• Host: E. coli
• Purity/Purification: >95 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5%Trehalose, 5% Mannitol, 0.01% Tween-80
• Sequence: Met 1-Ser 269

• Species: Human
• Tag: N-His
• Bio_activity: Measured by its binding ability in a functional ELISA. Immobilized human IL1B at 10 ug/mL (100 ul/well) can bind human IL1RThe EC50 of human IL1R1 is 0.198ug/mL.
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water(200ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Ships on blue ice. Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

SDS-PAGE

Related Product Information for IL-1 beta / IL1B active protein

Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).

NCBI and Uniprot Product Information

• NCBI GI #: 10835145
• NCBI GeneID: 3553
• NCBI Accession #: NP_000567.1
• NCBI GenBank Nucleotide #: NM_000576.2
• UniProt Accession #: P01584; Q53X59; Q53XX2; Q7M4S7; Q7RU01; Q96HE5; Q9UCT6
• Molecular Weight: Mol_Mass: 32.3 kDa; AP_Mol_Mass: 34 kDa
• NCBI Official Full Name: interleukin-1 beta proprotein
• NCBI Official Synonym Full Names: interleukin 1 beta
• NCBI Official Symbol: IL1B
• NCBI Official Synonym Symbols: IL-1; IL1F2; IL1-BETA
• NCBI Protein Information: interleukin-1 beta
• UniProt Protein Name: Interleukin-1 beta
• UniProt Gene Name: IL1B
• UniProt Synonym Gene Names: IL1F2; IL-1 beta

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q14.1

Cellular Component: cytosol; extracellular region; extracellular space

Molecular Function: cytokine activity; interleukin-1 receptor binding; protein domain specific binding

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; apoptosis; cell-cell signaling; cytokine and chemokine mediated signaling pathway; embryo implantation; hyaluronan biosynthetic process; inflammatory response; lipopolysaccharide-mediated signaling pathway; MAPKKK cascade; negative regulation of cell proliferation; negative regulation of insulin receptor signaling pathway; negative regulation of lipid catabolic process; negative regulation of lipid metabolic process; negative regulation of MAP kinase activity; positive regulation of angiogenesis; positive regulation of fever; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of heterotypic cell-cell adhesion; positive regulation of interferon-gamma production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of JNK cascade; positive regulation of lipid catabolic process; positive regulation of membrane protein ectodomain proteolysis; positive regulation of mitosis; positive regulation of NF-kappaB import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of phagocytosis; positive regulation of prostaglandin secretion; positive regulation of protein amino acid phosphorylation; positive regulation of T cell mediated immunity; positive regulation of T cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; positive regulation of vascular endothelial growth factor receptor signaling pathway; protein kinase B signaling cascade; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of insulin secretion; regulation of nitric-oxide synthase activity; sequestering of triacylglycerol; signal transduction

Disease: Gastric Cancer, Hereditary Diffuse