Growth Hormone Active Protein
Growth Hormone, Recombinant, Human (hGH)
Purity
Highly Purified98% by RP-HPLC or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Synonyms
Growth Hormone; Recombinant; Human (hGH); Growth Hormone active protein
Host
E Coli
Purity/Purification
Highly Purified
98% by RP-HPLC or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
98% by RP-HPLC or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Form/Format
Supplied as a lyophilized powder in mannitol and sodium phosphate buffer. Reconstitute in sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ile.
Biological Activity
Human Growth Hormone is fully biologically active when compared to World Health Organization (WHO) reference standard, which is 3u/mg.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.72 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Human Growth Hormone as a Reference Standard.
1. UV spectroscopy at 280nm using the absorbency value of 0.72 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Human Growth Hormone as a Reference Standard.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Growth Hormone active protein
GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.
Recombinant Human Growth Hormone is a single, non-glycosylated, polypeptide chain containing 192 amino acids and having a molecular mass of 22,260 Dalton.
Recombinant Human Growth Hormone is a single, non-glycosylated, polypeptide chain containing 192 amino acids and having a molecular mass of 22,260 Dalton.
Product Categories/Family for Growth Hormone active protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
22.26kD
NCBI Official Full Name
growth hormone
Protein Family
Uniprot Description
Growth hormone plays an important role in growth control and involved in the regulation of several anabolic processes.
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Product Notes
The Growth Hormone (Catalog #AAA637570) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pr o-Thr-Ile. It is sometimes possible for the material contained within the vial of "Growth Hormone, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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