growth hormone Active Protein | gh active protein
Fish (denis) growth hormone
Purity
The purity of recombinant Gilthead Seabream growth hormone is greater than 98.0% as determined by:. (a) Analysis by reducing and non-reducing SDS-PAGE gel.
(b) Gel-filtration chromatography under non denaturing conditions.
Synonyms
growth hormone; Fish (denis) growth hormone; Fish; gilthead seabream (Sparus aurata) growth hormone; gh active protein
Host
E Coli
Purity/Purification
The purity of recombinant Gilthead Seabream growth hormone is greater than 98.0% as determined by:
. (a) Analysis by reducing and non-reducing SDS-PAGE gel.
(b) Gel-filtration chromatography under non denaturing conditions.
. (a) Analysis by reducing and non-reducing SDS-PAGE gel.
(b) Gel-filtration chromatography under non denaturing conditions.
Form/Format
White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first ten N-terminal amino acids of recombinant Gilthead Seabream growth hormone was determined and was found to be Ala-Gln-Pro-Ile-Thr-Asp-Gly-Gln-Arg-Leu.
Sequence Length
204
Solubility
It is recommended to reconstitute the lyophilized recombinant Gilthead Seabream growth hormone in sterile 0.4% NaHCO3 adjusted, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation of recombinant Gilthead Seabream growth hormone was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.71 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences.
Dimers and Aggregates
The purified recombinant Gilthead Seabream growth hormone consists of > 97% monomers as determined by gel-filtration chromatography.
Endotoxin
Less than 0.1 ng/ug (IEU/ug) of recombinant Gilthead Seabream growth hormone.
Formulation
The recombinant Gilthead Seabream growth hormone was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.
Biological Activity
Binding assays of the 125I-labeled recombinant Gilthead Seabream growth hormone to dolphin fish liver microsomal fraction resulted in high specific binding characterized by a Ka of 1.93 nM and a Bmax of 540 fmol/mg microsomal fraction protein. Recombinant Gilthead Seabream growth hormone like ovine placental lactogen, exhibited growth-stimulating activity when applied orally to S.aurata larvae or intraperitoneally to juvenile fish.
Preparation and Storage
Lyophilized recombinant Gilthead Seabream growth hormone although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution of recombinant Gilthead Seabream growth hormone at > 0.1 mg/ml and up to 2 2 mg/ml and filter sterilization recombinant Gilthead Seabream growth hormone can be stored at +4 degree C.
Related Product Information for gh active protein
Recombinant Gilthead Seabream (Sparus aurata) growth hormone (gsGH) produced in E. coli (21.4 K) is a single, non-glycosylated, polypeptide chain containing 188 amino acids and having a molecular mass of ~ 21.392 kDa. Recombinant Gilthead Seabream growth hormone was purified by chromatographic techniques, according to Ben-Atia et al., General and Comparative Endocrinology 113, 155 -164 (1999).
Product Categories/Family for gh active protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
23,105 Da
NCBI Official Full Name
growth hormone
UniProt Protein Name
Somatotropin
Protein Family
UniProt Gene Name
gh
UniProt Entry Name
SOMA_SPAAU
Uniprot Description
Growth hormone plays an important role in growth control and is involved in the regulation of several anabolic processes. Implicated as an osmoregulatory substance important for seawater adaptation.
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Product Notes
The growth hormone gh (Catalog #AAA400007) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first ten N-terminal amino acids of recombinan t Gilthead Seabream growth hormone was determined and was found to be Ala-Gln-Pr o-Ile-Thr- Asp-Gly-Gl n-Arg-Leu. It is sometimes possible for the material contained within the vial of "growth hormone, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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