Glutathione S Transferase A1-1 Human Active Protein | Gsta2 active protein
Glutathione S Transferase A1-1, Recombinant Human (GST)
Gene Names
Gsta2; Gsta1
Purity
>=95% as assessed by inspection on a Coomassie® Blue-stained SDS-PAGE gel.
Synonyms
Glutathione S Transferase A1-1 Human; Glutathione S Transferase A1-1; Recombinant Human (GST); Gsta2 active protein
Host
E Coli
Purity/Purification
>=95% as assessed by inspection on a Coomassie® Blue-stained SDS-PAGE gel.
Form/Format
Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 50mM sodium chloride, 1mM DTT, 5mM EDTA, 50% glycerol.
Concentration
2.09mg/mL total protein using the Bradford protein assay with BSA as a standard. (varies by lot)
Specific Activity
79.1 Units/mg using spectrophotometric determination of 1-chloro-2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1 mM) in 100mM NaPO4 (pH 6.5) at room temperature.
Note
This purified product exhibits high enzymatic activity for CDNB, the synthetic substrate that is most commonly used for GST analyses. However, as is widely known for GST activity analysis using CDNB as a substrate, low concentrations (high dilutions) of the enzyme result in lower activity values. In contrast, initial velocities are much higher for more concentrated levels of enzyme but the rate decreases rapidly. These considerations should provide guidance when assaying this product under low concentrations or when using substrates other than CDNB.
Preparation and Storage
Ships on dry ice. Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for Gsta2 active protein
Human recombinant protein produced from overexpressing plasmid in E. coli.
Product Categories/Family for Gsta2 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23kD
NCBI Official Full Name
Glutathione S-transferase alpha-1
NCBI Official Synonym Full Names
glutathione S-transferase alpha 2
NCBI Official Symbol
Gsta2
NCBI Official Synonym Symbols
Gsta1
NCBI Protein Information
glutathione S-transferase alpha-1; GST B; GST 1-1; GST Ya1; GST A1-1; ligandin; GST 1a-1a; glutathione S-transferase A2; glutathione S-transferase Ya-1; glutathione S-transferase Ya subunit; glutathione-S-transferase, alpha type2; Glutathione-S-transferase alpha type (Yc?)
UniProt Protein Name
Glutathione S-transferase alpha-1
Protein Family
UniProt Gene Name
Gsta1
UniProt Synonym Gene Names
GST Ya1
UniProt Entry Name
GSTA1_RAT
NCBI Description
mRNA expression is induced by phenobarbital and by trans-stilbene oxide [RGD, Feb 2006]
Uniprot Description
GSTA5: Belongs to the GST superfamily. Alpha family. Homodimer
Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Transferase; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 2.5.1.18; Nuclear envelope; Other Amino Acids Metabolism - glutathione
Cellular Component: nuclear outer membrane; nucleus; cytosol
Molecular Function: glutathione binding; glutathione transferase activity; drug binding
Biological Process: metabolic process; aging; xenobiotic catabolic process
Research Articles on Gsta2
Similar Products
Product Notes
The Gsta2 gsta1 (Catalog #AAA636042) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Glutathione S Transferase A1-1 Human, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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